In vivo protein crystallization opens new routes in structural biology

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MARC

Koopmann, Rudolf (author)

In vivo protein crystallization opens new routes in structural biology

Article/chapterEnglish2012

Publisher, publication year, extent ...

2012-01-29
Springer Science and Business Media LLC,2012
printrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-169657
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-169657URI
https://doi.org/10.1038/nmeth.1859DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Subject headings and genre

NATURVETENSKAP Biologi Strukturbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Structural Biology hsv//eng
NATURVETENSKAP Biologi Biofysik hsv//swe
NATURAL SCIENCES Biological Sciences Biophysics hsv//eng

Added entries (persons, corporate bodies, meetings, titles ...)

Cupelli, Karolina (author)
Redecke, Lars (author)
Nass, Karol (author)
DePonte, Daniel P (author)
White, Thomas A (author)
Stellato, Francesco (author)
Rehders, Dirk (author)
Liang, Mengning (author)
Andreasson, JakobUppsala universitet,Molekylär biofysik(Swepub:uu)jakan998 (author)
Aquila, Andrew (author)
Bajt, Sasa (author)
Barthelmess, Miriam (author)
Barty, Anton (author)
Bogan, Michael J (author)
Bostedt, Christoph (author)
Boutet, Sebastien (author)
Bozek, John D (author)
Caleman, Carl (author)
Coppola, Nicola (author)
Davidsson, JanUppsala universitet,Fysikalisk kemi(Swepub:uu)jakan998 (author)
Doak, R Bruce (author)
Ekeberg, TomasUppsala universitet,Molekylär biofysik(Swepub:uu)tomek611 (author)
Epp, Sascha W (author)
Erk, Benjamin (author)
Fleckenstein, Holger (author)
Foucar, Lutz (author)
Graafsma, Heinz (author)
Gumprecht, Lars (author)
Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd (author)
Hampton, Christina Y (author)
Hartmann, Andreas (author)
Hartmann, Robert (author)
Hauser, Gunter (author)
Hirsemann, Helmut (author)
Holl, Peter (author)
Hunter, Mark S (author)
Kassemeyer, Stephan (author)
Kirian, Richard A (author)
Lomb, Lukas (author)
Maia, Filipe R N C (author)
Kimmel, Nils (author)
Martin, Andrew V (author)
Messerschmidt, Marc (author)
Reich, Christian (author)
Rolles, Daniel (author)
Rudek, Benedikt (author)
Rudenko, Artem (author)
Schlichting, Ilme (author)
Schulz, Joachim (author)
Seibert, M MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255 (author)
Shoeman, Robert L (author)
Sierra, Raymond G (author)
Soltau, Heike (author)
Stern, Stephan (author)
Struder, Lothar (author)
Timneanu, NicusorUppsala universitet,Molekylär biofysik(Swepub:uu)nti19847 (author)
Ullrich, Joachim (author)
Wang, Xiaoyu (author)
Weidenspointner, Georg (author)
Weierstall, Uwe (author)
Williams, Garth J (author)
Wunderer, Cornelia B (author)
Fromme, Petra (author)
Spence, John C H (author)
Stehle, Thilo (author)
Chapman, Henry N (author)
Betzel, Christian (author)
Duszenko, Michael (author)
Uppsala universitetMolekylär biofysik (creator_code:org_t)

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In:Nature Methods: Springer Science and Business Media LLC9:3, s. 259-2621548-70911548-7105

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By the author/editor: Koopmann, Rudolf; Cupelli, Karolin ...; Redecke, Lars; Nass, Karol; DePonte, Daniel ...; White, Thomas A; show more...; Stellato, France ...; Rehders, Dirk; Liang, Mengning; Andreasson, Jako ...; Aquila, Andrew; Bajt, Sasa; Barthelmess, Mir ...; Barty, Anton; Bogan, Michael J; Bostedt, Christo ...; Boutet, Sebastie ...; Bozek, John D; Caleman, Carl; Coppola, Nicola; Davidsson, Jan; Doak, R Bruce; Ekeberg, Tomas; Epp, Sascha W; Erk, Benjamin; Fleckenstein, Ho ...; Foucar, Lutz; Graafsma, Heinz; Gumprecht, Lars; Hajdu, Janos; Hampton, Christi ...; Hartmann, Andrea ...; Hartmann, Robert; Hauser, Gunter; Hirsemann, Helmu ...; Holl, Peter; Hunter, Mark S; Kassemeyer, Step ...; Kirian, Richard ...; Lomb, Lukas; Maia, Filipe R N ...; Kimmel, Nils; Martin, Andrew V; Messerschmidt, M ...; Reich, Christian; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilm ...; Schulz, Joachim; Seibert, M Marvi ...; Shoeman, Robert ...; Sierra, Raymond ...; Soltau, Heike; Stern, Stephan; Struder, Lothar; Timneanu, Nicuso ...; Ullrich, Joachim; Wang, Xiaoyu; Weidenspointner, ...; Weierstall, Uwe; Williams, Garth ...; Wunderer, Cornel ...; Fromme, Petra; Spence, John C H; Stehle, Thilo; Chapman, Henry N; Betzel, Christia ...; Duszenko, Michae ...; show less...

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NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Structural Biolo ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

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By the university: Uppsala University

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