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Sensitive detection...
Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
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Dahlén, P (author)
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Syvänen, Ann-Christine (author)
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Hurskainen, P (author)
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Kwiatkowski, M (author)
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Sund, C (author)
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Ylikoski, J (author)
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Söderlund, H (author)
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Lövgren, T (author)
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- Elsevier BV, 1987
- 1987
- English.
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In: Molecular and Cellular Probes. - : Elsevier BV. - 0890-8508 .- 1096-1194. ; 1:2, s. 159-168
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Europium has been used as a non-radioactive marker in immunoassays as this metal can be detected with high sensitivity by time-resolved fluorometry. In this work streptavidin labeled with europium was used to detect biotinylated probes in a sandwich nucleic-acid hybridization assay with microtitration strips as the solid phase. pBR 322 plasmids were detected with a sensitivity of 4 × 105 molecules. As the sample is added in solution in sandwich hybridization, fast and simple sample pre-treatment can be used without encountering background problems. The method was applied to test bacterial samples of uropathogenic Escherichia coli strains for the presence of the β-lactamase gene.
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- ref (subject category)
- art (subject category)
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