onr:"swepub:oai:DiVA.org:uu-170295"
Search: onr:"swepub:oai:DiVA.org:uu-170295" > Kinetics of GADD45a...
- 1 of 1
- Previous record
- Next record
- To hitlist
Kinetics of GADD45a, TP53 and CASP3 gene expression in the rat lens in vivo in response to exposure to double threshold dose of UV-B radiation
-
- Galichanin, Konstantin (author)
- Karolinska Institutet,Uppsala universitet,Oftalmiatrik
-
- Svedlund, Jessica (author)
- Uppsala universitet,Endokrinkirurgi
-
- Söderberg, Per (author)
- Uppsala universitet,Oftalmiatrik
- (creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
- In: Experimental Eye Research. - : Elsevier BV. - 0014-4835 .- 1096-0007. ; 97:1, s. 19-23
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- The purpose of the present study was to investigate the evolution of expression of mRNA message for the genes for the genome stress sensor GADD45α, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens in vivo in response to exposure to UVR around 300 nm. Forty six week old female albino Sprague-Dawley rats were unilaterally exposed to double threshold dose for cataract induction, 8 kJ/m2 (8.9 W/m2 for 15 min), of UVR (λmax = 300 nm). The animals were sacrificed at 1, 5, 24 and 120 h following exposure to UVR-B. For each of the GADD45α, TP53 and CASP3 genes, respectively, mRNA expression in the lenses was measured by quantitative RT-PCR. It was found that expression of mRNA for GADD45α transiently increases between 5 and 24 h after exposure. TP53 is slightly downregulated in exposed lenses at 1 and 5 h after exposure and thereafter the mRNA expression increases with a constant rate of 9.4∗10−3 rel. units/h to a 1.8 fold increase at 120 h after exposure. Expression of mRNA for CASP3 is downregulated at 1, 5 and 24 h after in vivo exposure and then increases with a constant rate of 4.7∗10−3 rel. units/h, upto a 1.3 fold upregulation at 120 h. Double threshold dose of UVR, for short delay onset of cataract, in vivo causes a transient upregulation of the stress sensor GADD45α, a concurrent downregulation of TP53 and CASP3, followed by a constant upregulation of TP53 that precedes a constant upregulation of CASP3.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oftalmologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Ophthalmology (hsv//eng)
Keyword
- lens
- UVR
- stress sensing
- GADD45α
- TP53
- CASP3
- apoptosis
- Ophtalmology
- Oftalmiatrik
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Experimental Eye Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Galichanin, Kons ...
- Svedlund, Jessic ...
- Söderberg, Per
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Ophthalmology
- Articles in the publication
- Experimental Eye ...
- By the university
- Uppsala University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
