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Antibody-based Prot...
Antibody-based Protein Profiling of the Human Chromosome 21
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- Uhlén, Mathias (author)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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- Oksvold, Per (author)
- KTH,Proteomik
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- Älgenäs, Cajsa (author)
- KTH,Proteomik
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- Hamsten, Carl (author)
- KTH,Proteomik
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- Fagerberg, Linn (author)
- KTH,Proteomik
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- Klevebring, Daniel (author)
- Department of Medical Epidemiology, Karolinska Institute
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- Lundberg, Emma (author)
- KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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- Odeberg, Jacob (author)
- Karolinska Institutet,KTH,Proteomik,Science for Life Laboratory, SciLifeLab
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- Pontén, Fredrik (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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Kondo, Tadashi (author)
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- Sivertsson, Åsa (author)
- KTH,Proteomik
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(creator_code:org_t)
- 2012
- 2012
- English.
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In: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 11:3
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- The Human Proteome Project has been proposed to create a knowledge-based resource based on a systematical mapping of all human proteins, chromosome by chromosome, in a gene-centric manner. With this background, we here describe the systematic analysis of chromosome 21 using an antibody-based approach for protein profiling using both confocal microscopy and immunohistochemistry, complemented with transcript profiling using next generation sequencing data. We also describe a new approach for protein isoform analysis using a combination of antibody-based probing and isoelectric focusing. The analysis has identified several genes on chromosome 21 with no previous evidence on the protein level, and the isoform analysis indicates that a large fraction of human proteins have multiple isoforms. A chromosome-wide matrix is presented with status for all chromosome 21 genes regarding subcellular localization, tissue distribution, and molecular characterization of the corresponding proteins. The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Keyword
- IBD
- colitis
- Pathology
- Patologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Uhlén, Mathias
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Oksvold, Per
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Älgenäs, Cajsa
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Hamsten, Carl
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Fagerberg, Linn
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Klevebring, Dani ...
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show more...
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Lundberg, Emma
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Odeberg, Jacob
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Pontén, Fredrik
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Kondo, Tadashi
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Sivertsson, Åsa
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Clinical Laborat ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
- Articles in the publication
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Molecular & Cell ...
- By the university
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Uppsala University
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Royal Institute of Technology
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Karolinska Institutet