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  • Uhlén, MathiasKTH,Proteomik,Science for Life Laboratory, SciLifeLab (author)

Antibody-based Protein Profiling of the Human Chromosome 21

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • 2012
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-171237
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-171237URI
  • https://doi.org/10.1074/mcp.M111.013458DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-51404URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:124247735URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QS 20111212
  • The Human Proteome Project has been proposed to create a knowledge-based resource based on a systematical mapping of all human proteins, chromosome by chromosome, in a gene-centric manner. With this background, we here describe the systematic analysis of chromosome 21 using an antibody-based approach for protein profiling using both confocal microscopy and immunohistochemistry, complemented with transcript profiling using next generation sequencing data. We also describe a new approach for protein isoform analysis using a combination of antibody-based probing and isoelectric focusing. The analysis has identified several genes on chromosome 21 with no previous evidence on the protein level, and the isoform analysis indicates that a large fraction of human proteins have multiple isoforms. A chromosome-wide matrix is presented with status for all chromosome 21 genes regarding subcellular localization, tissue distribution, and molecular characterization of the corresponding proteins. The path to generate a chromosome-specific resource, including integrated data from complementary assay platforms, such as mass spectrometry and gene tagging analysis, is discussed.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Oksvold, PerKTH,Proteomik(Swepub:kth)u1qey3lv (author)
  • Älgenäs, CajsaKTH,Proteomik(Swepub:kth)u1v0vamk (author)
  • Hamsten, CarlKTH,Proteomik(Swepub:kth)u1r44ozl (author)
  • Fagerberg, LinnKTH,Proteomik(Swepub:kth)u1dglfid (author)
  • Klevebring, DanielDepartment of Medical Epidemiology, Karolinska Institute(Swepub:kth)u10ab3gt (author)
  • Lundberg, EmmaKTH,Proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u12bylj1 (author)
  • Odeberg, JacobKarolinska Institutet,KTH,Proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1e4yljo (author)
  • Pontén, FredrikUppsala universitet,Institutionen för immunologi, genetik och patologi(Swepub:uu)fredpont (author)
  • Kondo, Tadashi (author)
  • Sivertsson, ÅsaKTH,Proteomik(Swepub:kth)u195d5b0 (author)
  • KTHProteomik (creator_code:org_t)

Related titles

  • In:Molecular & Cellular Proteomics11:31535-94761535-9484

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