Sökning: onr:"swepub:oai:DiVA.org:uu-17175" >
Replication of Ljun...
Replication of Ljungan virus in cell culture : the genomic 5'-end, infectious cDNA clones and host cell response to viral infections
-
- Ekström, Jens-Ola (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
-
- Tolf, Conny (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
-
- Fahlgren, Camilla (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
-
visa fler...
-
- Johansson, Susanne (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
-
- Arbrandt, Gustav (författare)
- Apodemus, Stockholm
-
- Niklasson, Bo (författare)
- Apodemus, Stockholm
-
- Edman, Kjell (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
-
- Lindberg, A. Michael (författare)
- Högskolan i Kalmar,Naturvetenskapliga institutionen
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
-
Ingår i: Virus Research. - : Elsevier BV. - 0168-1702 .- 1872-7492. ; 130:1-2, s. 129-139
- Relaterad länk:
-
http://www.ncbi.nlm....
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Ljungan virus (LV) is a picornavirus recently isolated from bank voles (Clethrionomys glareolus). The previously uncharacterised 5'-end sequence of the LV genome was determined. Infectious cDNA clones were constructed of the wild type LV prototype strain 87-012 and of the cytolytically replicating cell culture adapted variant 87-012G. Virus generated from cDNA clones showed identical growth characteristics as uncloned virus stocks. Cell culture adapted LV, 87-012G, showed a clear cytopathic effect (CPE) at 3-4 days post-infection (p.i.). Virus titers, determined by plaque titration, increased however only within the first 18h p.i. Replication of LV (+) strand RNA was determined by real-time PCR and corresponded in time with increasing titers. In contrast, the amounts of the replication intermediate, the (-) strand, continued to increase until the cells showed CPE. This indicates separate controlling mechanisms for replication of LV (+) and (-) genome strands. Replication was also monitored by immunofluorescence (IF) staining. IF staining of both prototype 87-012 and the CPE causing 87-012G showed groups of 5-25 infected cells at 48h p.i., suggesting a, for picornaviruses, not previously described direct cell-to-cell transmission.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- 5' Untranslated Regions/genetics
- Animals
- Cell Line
- Cercopithecus aethiops
- Cytopathogenic Effect; Viral
- DNA; Complementary/genetics
- Parechovirus/*growth & development
- Plaque Assay
- RNA; Viral/biosynthesis/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis
- Virus Cultivation/*methods
- Virus Replication
- Biomedical Sciences
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas