Automated sample preparation method for mass spectrometry analysis on recombinant proteins

• A completely automated procedure for the purification and desalting of proteins with a polyhistidine purification tag prior to mass spectrometry analysis is presented. The system is ideal for rapid quality control and optimization studies and it provides researchers with a straightforward, reliable tool for studies of recombinant proteins. Forty-eight samples can be prepared within 4.5 h and only small cultivation and buffer volumes are needed. In this proof of concept, 19,000-35,000 Da recombinant proteins from both crude and clarified cell lysates were successfully prepared for subsequent analysis by electrospray ionization anti matrix-assisted laser desorption/ionization mass spectrometry as well as by gel electrophoresis.

Keyword

Mass spectrometry

High-throughput

Automated sample preparation

Recombinant proteins

IMAC

RPLC

affinity-chromatography

purification

Publication and Content Type

ref (subject category)

art (subject category)