Human cathepsin G lacking functional glycosylation site is proteolytically processed and targeted for storage in granules after transfection to the rat basophilic/mast cell line RBL or the murine myeloid cell line 32D

Keyword

Amino Acid Sequence

Animals

Base Sequence

Cathepsins/*biosynthesis/isolation & purification

Cell Line

Chromatography; Affinity

Cytoplasmic Granules/*metabolism

DNA Primers

Glutamine

Glycosylation

Humans

Kinetics

Leukemia; Basophilic; Acute

Mast Cells

Mice

Molecular Sequence Data

Mutagenesis; Site-Directed

Point Mutation

Polymerase Chain Reaction

Protein Processing; Post-Translational

Rats

Recombinant Proteins/biosynthesis/isolation & purification

Sequence Deletion

Serine Endopeptidases

Transfection

Tumor Cells; Cultured

Publication and Content Type

ref (subject category)

art (subject category)