Search: onr:"swepub:oai:DiVA.org:uu-175309" >
GABA-A Channel Subu...
-
Smits, AnjaUppsala universitet,Neurologi
(author)
GABA-A Channel Subunit Expression in Human Glioma Correlates with Tumor Histology and Clinical Outcome
- Article/chapterEnglish2012
Publisher, publication year, extent ...
-
2012-05-17
-
Public Library of Science (PLoS),2012
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-175309
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-175309URI
-
https://doi.org/10.1371/journal.pone.0037041DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:124815072URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
GABA (γ-aminobutyric acid) is the main inhibitory neurotransmitter in the CNS and is present in high concentrations in presynaptic terminals of neuronal cells. More recently, GABA has been ascribed a more widespread role in the control of cell proliferation during development where low concentrations of extrasynaptic GABA induce a tonic activation of GABA receptors. The GABA-A receptor consists of a ligand-gated chloride channel, formed by five subunits that are selected from 19 different subunit isoforms. The functional and pharmacological properties of the GABA-A channels are dictated by their subunit composition. Here we used qRT-PCR to compare mRNA levels of all 19 GABA-A channel subunits in samples of human glioma (n = 29) and peri-tumoral tissue (n = 5). All subunits except the ρ1 and ρ3 subunit were consistently detected. Lowest mRNA levels were found in glioblastoma compared to gliomas of lower malignancy, except for the θ subunit. The expression and cellular distribution of the α1, γ1, ρ2 and θ subunit proteins was investigated by immunohistochemistry on tissue microarrays containing 87 gliomas grade II. We found a strong co-expression of ρ2 and θ subunits in both astrocytomas (r = 0.86, p<0.0001) and oligodendroglial tumors (r = 0.66, p<0.0001). Kaplan-Meier analysis and Cox proportional hazards modeling to estimate the impact of GABA-A channel subunit expression on survival identified the ρ2 subunit (p = 0.043) but not the θ subunit (p = 0.64) as an independent predictor of improved survival in astrocytomas, together with established prognostic factors. Our data give support for the presence of distinct GABA-A channel subtypes in gliomas and provide the first link between specific composition of the A-channel and patient survival.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Jin, ZheUppsala universitet,Fysiologi(Swepub:uu)zheji315
(author)
-
Elsir, TamadorUppsala universitet,Neurologi(Swepub:uu)elsta774
(author)
-
Pedder, HugoUppsala universitet,Neurologi
(author)
-
Nistér, MonicaKarolinska Institutet
(author)
-
Alafuzoff, IrinaUppsala universitet,Molekylär och morfologisk patologi(Swepub:uu)irial548
(author)
-
Dimberg, AnnaUppsala universitet,Cancer och vaskulärbiologi(Swepub:uu)annadimb
(author)
-
Edqvist, Per-HenrikUppsala universitet,Molekylär och morfologisk patologi(Swepub:uu)peedq227
(author)
-
Pontén, FredrikUppsala universitet,Molekylär och morfologisk patologi(Swepub:uu)fredpont
(author)
-
Aronica, Eleonora
(author)
-
Birnir, BryndisUppsala universitet,Fysiologi(Swepub:uu)brybi979
(author)
-
Uppsala universitetNeurologi
(creator_code:org_t)
Related titles
-
In:PLOS ONE: Public Library of Science (PLoS)7:5, s. e37041-1932-6203
Internet link
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Smits, Anja
-
Jin, Zhe
-
Elsir, Tamador
-
Pedder, Hugo
-
Nistér, Monica
-
Alafuzoff, Irina
-
show more...
-
Dimberg, Anna
-
Edqvist, Per-Hen ...
-
Pontén, Fredrik
-
Aronica, Eleonor ...
-
Birnir, Bryndis
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Neurology
- Articles in the publication
-
PLOS ONE
- By the university
-
Uppsala University
-
Karolinska Institutet