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Next generation RNA...
Next generation RNA-sequencing in prognostic subsets of chronic lymphocytic leukemia
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- Mansouri, Larry (author)
- Uppsala universitet,Hematologi och immunologi,Rosenquist Brandell
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- Gunnarsson, Rebeqa (author)
- Uppsala universitet,Hematologi och immunologi,Rosenquist Brandell
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- Sutton, Lesley-Ann (author)
- Uppsala universitet,Hematologi och immunologi,Rosenquist Brandell
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- Ameur, Adam (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Hooper, Sean D. (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Mayrhofer, Markus (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin,Science for Life Laboratory, SciLifeLab
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- Juliusson, Gunnar (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine
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- Isaksson, Anders (author)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin,Science for Life Laboratory, SciLifeLab
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- Gyllensten, Ulf (author)
- Uppsala universitet,Genomik,Gyllensten
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- Rosenquist, Richard (author)
- Uppsala universitet,Hematologi och immunologi,Rosenquist Brandell
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(creator_code:org_t)
- 2012-06-03
- 2012
- English.
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In: American Journal of Hematology. - : Wiley. - 0361-8609 .- 1096-8652. ; 87:7, s. 737-740
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Abstract
Subject headings
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- Advances in next-generation RNA-sequencing have revealed the complexity of transcriptomes by allowing both coding and noncoding (nc) RNAs to be analyzed. However, limited data exist regarding the whole transcriptional landscape of chronic lymphocytic leukemia (CLL). In this pilot-study, we evaluated RNA-sequencing in CLL by comparing two subsets which carry almost identical or `` stereotyped'' B-cell receptors with distinct clinical outcome, that is the poor-prognostic subset # 1 (n = 4) and the more favorable-prognostic subset # 4 (n = 4). Our analysis revealed that 156 genes (e.g. LPL, WNT9A) and 76 ncRNAs, (e. g. SNORD48, SNORD115) were differentially expressed between the subsets. This technology also enabled us to identify numerous subset-specific splice variants (n = 406), which were predominantly expressed in subset # 1, including a splice-isoform of MSI2 with a novel start exon. A further important application of RNA-sequencing was for mutation detection and revealed 16-30 missense mutations per sample; notably many of these changes were found in genes with a strong potential for involvement in CLL pathogenesis, e. g., ATM and NOTCH2. This study not only demonstrates the effectiveness of RNA-sequencing for identifying mutations, quantifying gene expression and detecting splicing events, but also highlights the potential such global approaches have to significantly advance our understanding of the molecular mechanisms behind CLL development.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Keyword
- LPL expression
- LPL catalytical activity
- IGHV mutational status
- IGHV3 21 usage
- Chronic lymphocytic leukemia
- Prognosis
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Mansouri, Larry
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Gunnarsson, Rebe ...
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Sutton, Lesley-A ...
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Ameur, Adam
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Hooper, Sean D.
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Mayrhofer, Marku ...
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show more...
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Juliusson, Gunna ...
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Isaksson, Anders
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Gyllensten, Ulf
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Rosenquist, Rich ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Hematology
- Articles in the publication
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American Journal ...
- By the university
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Uppsala University
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Lund University