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  • Hammar, PetterUppsala universitet,Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab (author)

The lac repressor displays facilitated diffusion in living cells

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • American Association for the Advancement of Science (AAAS),2012
  • printrdacarrier

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  • LIBRIS-ID:oai:DiVA.org:uu-176861
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-176861URI
  • https://doi.org/10.1126/science.1221648DOI

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  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Transcription factors (TFs) are proteins that regulate the expression of genes by binding sequence-specific sites on the chromosome. It has been proposed that to find these sites fast and accurately, TFs combine one-dimensional (1D) sliding on DNA with 3D diffusion in the cytoplasm. This facilitated diffusion mechanism has been demonstrated in vitro, but it has not been shown experimentally to be exploited in living cells. We have developed a single-molecule assay that allows us to investigate the sliding process in living bacteria. Here we show that the lac repressor slides 45 ± 10 base pairs on chromosomal DNA and that sliding can be obstructed by other DNA-bound proteins near the operator. Furthermore, the repressor frequently (>90%) slides over its natural lacO(1) operator several times before binding. This suggests a trade-off between rapid search on nonspecific sequences and fast binding at the specific sequence.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Leroy, PruneUppsala universitet,Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)myrle974 (author)
  • Mahmutovic, AnelUppsala universitet,Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)anema746 (author)
  • Marklund, Erik GUppsala universitet,Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)ermar499 (author)
  • Berg, Otto GUppsala universitet,Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)obe15246 (author)
  • Elf, JohanUppsala universitet,Beräknings- och systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)johaande (author)
  • Uppsala universitetBeräknings- och systembiologi (creator_code:org_t)

Related titles

  • In:Science: American Association for the Advancement of Science (AAAS)336:6088, s. 1595-15980036-80751095-9203

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By the author/editor
Hammar, Petter
Leroy, Prune
Mahmutovic, Anel
Marklund, Erik G
Berg, Otto G
Elf, Johan
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Science
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Uppsala University

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