Search: onr:"swepub:oai:DiVA.org:uu-182831" >
Hidden Diversity Re...
Hidden Diversity Revealed : Genomic, Transcriptomic and Functional Studies of Diplomonads
-
- Jerlström-Hultqvist, Jon, 1982- (author)
- Uppsala universitet,Mikrobiologi,Svärd Group
-
- Svärd, Staffan, Professor (thesis advisor)
- Uppsala universitet,Mikrobiologi
-
- Hehl, Adrian, Professor (opponent)
- University of Zürich, Institute of Parasitology
-
(creator_code:org_t)
- ISBN 9789155485207
- Uppsala : Acta Universitatis Upsaliensis, 2012
- English 104 s.
-
Series: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, 1651-6214 ; 990
- Related links:
-
https://uu.diva-port... (primary) (Raw object)
-
show more...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- The diplomonads are a diverse group of eukaryotic microbes found in oxygen limited environments such as the intestine of animals were they may cause severe disease. Among them, the prominent human parasite Giardia intestinalis non-invasively colonizes the small intestine of humans and animals where it induces the gastrointestinal disease giardiasis. Two of the eight genetic groups of G. intestinalis, assemblage A and B, are known to infect humans and have zoonotic potential. At the start of project, genome scale data from assemblage B-H was either sparse or entirely missing.In this thesis, genome sequencing was performed on the assemblage B isolate GS (Paper I) and the P15 isolate (Paper III) of the hoofed-animals specific assemblage E to investigate the underlying components of phenotypic diversity in Giardia. Comparisons to assemblage A isolate WB revealed large genomic differences; entirely different repertoires of surface antigens, genome rearrangements and isolate specific coding sequences of potential bacterial origin. We established that genomic differences are also manifested at the transcriptome level (Paper VIII). In a follow up analysis (Paper IV) we concluded that the Giardia assemblages are largely reproductively isolated. The large genomic differences observed between Giardia isolates can explain the phenotypic diversity of giardiasis.The adaptation of diplomonads was further studied in Spironucleus barkhanus (Paper II), a fish commensal of grayling, that is closely related to the fish pathogen Spironucleus salmonicida, causative agent of systemic spironucleosis in salmonid fish. We identified substantial genomic differences in the form of divergent genome size, primary sequence divergence and evidence of allelic sequence heterozygosity, a feature not seen in S. salmonicida.We devised a transfection system for S. salmonicida (Paper VI) and applied it to the study of the mitochondrial remnant organelle (Paper VII). Our analyses showed that S. salmonicida harbor a hydrogenosome, an organelle with more metabolic capabilities than the mitosome of Giardia. Phylogenetic reconstructions of key hydrogenosomal enzymes showed an ancient origin, indicating a common origin to the hydrogenosome in parabasilids and diplomonads.In conclusion, the thesis has provided important insights into the adaptation of diplomonads in the present and the distant past, revealing hidden diversity.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- Giardia intestinalis
- Spironucleus salmonicida
- Spironucleus barkhanus
- intestinal parasite
- hydrogenosome
- mitosome
- lateral gene transfer
- horizontal gene transfer
- diplomonad
- metamonad
- sexual recombination
- transfection
- protein complex purification
- Biology with specialization in Evolutionary Organismal Biology
- Biologi med inriktning mot evolutionär organismbiologi
- Biologi med inriktning mot mikrobiologi
- Biology with specialization in Microbiology
- Biology with specialization in Molecular Biology
- Biologi med inriktning mot molekylärbiologi
- Biologi med inriktning mot molekylär evolution
- Biology with specialization in Molecular Evolution
Publication and Content Type
- vet (subject category)
- dok (subject category)
Find in a library
To the university's database