onr:"swepub:oai:DiVA.org:uu-184896"
Search: onr:"swepub:oai:DiVA.org:uu-184896" > A site-specific foc...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Rubino, StefanoUppsala universitet,Tillämpad materialvetenskap (author)
A site-specific focused-ion-beam lift-out method for cryo Transmission Electron Microscopy
- Article/chapterEnglish2012
Publisher, publication year, extent ...
- Elsevier BV,2012
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-184896
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-184896URI
- https://doi.org/10.1016/j.jsb.2012.08.012DOI
- https://res.slu.se/id/publ/42408URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- De två första författarna delar förstaförfattarskapet.
- The focused-ion-beam (FIB) is the method of choice for site-specific sample preparation for Transmission Electron Microscopy (TEM) in material sciences. A lamella can be physically lifted out from a specific region of a bulk specimen with submicrometer precision and thinned to electron transparency for high-resolution imaging in the TEM. The possibility to use this tool in life sciences applications has been limited by the lack of lift-out capabilities at the cryogenic temperatures often needed for biological samples. Conventional cryo-TEM sample preparation is mostly based on ultramicrotomy, a procedure that is not site-specific and known to produce artifacts. Here we demonstrate how a cooled nanomanipulator and a custom-built transfer station can be used to achieve cryo-preparation of TEM samples with the FIB, enabling high-resolution investigation of frozen-hydrated specimens in the TEM.
Subject headings and genre
- NATURVETENSKAP Biologi Mikrobiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
- Cryo-EM
- Cryo-FIB
- Cryo-SEM
- Cryo-TEM
- FIB
- Frozen-hydrated sample
- Sample preparation
- Specimen thinning
- TEM
- Teknisk fysik med inriktning mot materialvetenskap
- Engineering Science with specialization in Materials Science
Added entries (persons, corporate bodies, meetings, titles ...)
- Akhtar, SultanUppsala universitet,Tillämpad materialvetenskap(Swepub:uu)sulak695 (author)
- Melin, PetterSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Dept of Microbiology, Swedish University of Agricultural Sciences, Uppsala(Swepub:slu)50397 (author)
- Searle, AndrewGatan Inc, Abingdon Oxon, UK (author)
- Spellward, PaulGatan Inc, Abingdon Oxon, UK (author)
- Leifer, KlausUppsala universitet,Tillämpad materialvetenskap(Swepub:uu)kllei839 (author)
- Uppsala universitetTillämpad materialvetenskap (creator_code:org_t)
- Sveriges lantbruksuniversitet
Related titles
- In:Journal of Structural Biology: Elsevier BV180:3, s. 572-5761047-84771095-8657
Internet link
Find in a library
- Journal of Structural Biology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Rubino, Stefano
- Akhtar, Sultan
- Melin, Petter
- Searle, Andrew
- Spellward, Paul
- Leifer, Klaus
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Microbiology
- Articles in the publication
- Journal of Struc ...
- By the university
- Uppsala University
- Swedish University of Agricultural Sciences
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
