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Paralemmin-1 is ove...
Paralemmin-1 is over-expressed in estrogen-receptor positive breast cancers
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Turk, Casey M. (författare)
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Fagan-Solis, Katerina D. (författare)
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Williams, Kristin E. (författare)
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Gozgit, Joseph M. (författare)
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Smith-Schneider, Sallie (författare)
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Marconi, Sharon A. (författare)
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Otis, Christopher N. (författare)
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Crisi, Giovanna M. (författare)
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Anderton, Douglas L. (författare)
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- Kilimann, Manfred W. (författare)
- Uppsala universitet,Molekylär cellbiologi
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Arcaro, Kathleen F. (författare)
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(creator_code:org_t)
- 2012-05-10
- 2012
- Engelska.
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Ingår i: Cancer Cell International. - : Springer Science and Business Media LLC. - 1475-2867. ; 12, s. 17-
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://cancerci.bio...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Paralemmin-1 is a phosphoprotein lipid-anchored to the cytoplasmic face of membranes where it functions in membrane dynamics, maintenance of cell shape, and process formation. Expression of paralemmin-1 and its major splice variant (Delta exon 8) as well as the extent of posttranslational modifications are tissue-and development-specific. Paralemmin-1 expression in normal breast and breast cancer tissue has not been described previously. Results: Paralemmin-1 mRNA and protein expression was evaluated in ten breast cell lines, 26 primary tumors, and 10 reduction mammoplasty (RM) tissues using real time RT-PCR. Paralemmin-1 splice variants were assessed in tumor and RM tissues using a series of primers and RT-PCR. Paralemmin-1 protein expression was examined in cell lines using Western Blots and in 31 ductal carcinomas in situ, 65 infiltrating ductal carcinomas, and 40 RM tissues using immunohistochemistry. Paralemmin-1 mRNA levels were higher in breast cancers than in RM tissue and estrogen receptor (ER)-positive tumors had higher transcript levels than ER-negative tumors. The Delta exon 8 splice variant was detected more frequently in tumor than in RM tissues. Protein expression was consistent with mRNA results showing higher paralemmin-1 expression in ER-positive tumors. Conclusions: The differential expression of paralemmin-1 in a subset of breast cancers suggests the existence of variation in membrane dynamics that may be exploited to improve diagnosis or provide a therapeutic target.
Nyckelord
- Paralemmin-1
- Breast cancer
- PALM
- Estrogen receptor
- Tissue microarrays
- Splice variants
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Turk, Casey M.
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Fagan-Solis, Kat ...
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Williams, Kristi ...
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Gozgit, Joseph M ...
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Smith-Schneider, ...
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Marconi, Sharon ...
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visa fler...
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Otis, Christophe ...
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Crisi, Giovanna ...
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Anderton, Dougla ...
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Kilimann, Manfre ...
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Arcaro, Kathleen ...
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visa färre...
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Cancer Cell Inte ...
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Uppsala universitet