Search: onr:"swepub:oai:DiVA.org:uu-191767" >
Full-Length Minor A...
Full-Length Minor Ampullate Spidroin Gene Sequence
- Article/chapterEnglish2012
Publisher, publication year, extent ...
-
2012-12-14
-
Public Library of Science (PLoS),2012
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-191767
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-191767URI
-
https://doi.org/10.1371/journal.pone.0052293DOI
-
https://res.slu.se/id/publ/42660URI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:125824263URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Spider silk includes seven protein based fibers and glue-like substances produced by glands in the spider's abdomen. Minor ampullate silk is used to make the auxiliary spiral of the orb-web and also for wrapping prey, has a high tensile strength and does not supercontract in water. So far, only partial cDNA sequences have been obtained for minor ampullate spidroins (MiSps). Here we describe the first MiSp full-length gene sequence from the spider species Araneus ventricosus, using a multidimensional PCR approach. Comparative analysis of the sequence reveals regulatory elements, as well as unique spidroin gene and protein architecture including the presence of an unusually large intron. The spliced full-length transcript of MiSp gene is 5440 bp in size and encodes 1766 amino acid residues organized into conserved nonrepetitive N- and C-terminal domains and a central predominantly repetitive region composed of four units that are iterated in a non regular manner. The repeats are more conserved within A. ventricosus MiSp than compared to repeats from homologous proteins, and are interrupted by two nonrepetitive spacer regions, which have 100% identity even at the nucleotide level.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Liu, Xiangqin
(author)
-
Zhang, Yunlong
(author)
-
Lin, Senzhu
(author)
-
Yang, Zijiang
(author)
-
Johansson, JanKarolinska Institutet,Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute,Tallinn University,institutionen för anatomi, fysiologi och biokemi - afb (slu), avdelningen för biokemi - (slu)(Swepub:slu)47966
(author)
-
Rising, AnnaKarolinska Institutet,Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute,institutionen för anatomi, fysiologi och biokemi - afb (slu), avdelningen för biokemi - (slu)(Swepub:slu)47422
(author)
-
Meng, Qing
(author)
-
Sveriges lantbruksuniversitetInstitutionen för anatomi, fysiologi och biokemi
(creator_code:org_t)
-
Sveriges lantbruksuniversitet
Related titles
-
In:PLOS ONE: Public Library of Science (PLoS)7:12, s. e52293-1932-6203
Internet link
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database