PCR detection of haemophilus influenzae from respiratory specimens

• The detection of Haemophilus influenzae by conventional methods like culture is time-consuming and may give false-negative results, especially during ongoing antibiotic treatment. Therefore, non-culture based methods that are sensitive, specific, and rapid are valuable for early diagnosis and effective therapy. Here we describe a quantitative real-time PCR assay based on the outer membrane P6 gene omp6, to detect H. influenzae and its application on respiratory tract specimens.

Keyword

Haemophilus influenzae

Outer membrane P6

Pneumonia

Quantitative PCR

Real-time PCR

Publication and Content Type

ref (subject category)

kap (subject category)