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Integration of clin...
Integration of clinical data with a genome-scale metabolic model of the human adipocyte
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- Mardinoglu, Adil, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ågren, Rasmus, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Kampf, Caroline (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Uppsala University
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- Asplund, Anna (author)
- Uppsala universitet,Molekylär och morfologisk patologi,Science for Life Laboratory, SciLifeLab,Uppsala University,Hammersmith Hospital
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- Nookaew, Intawat, 1977 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Jacobson, Peter, 1962 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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Walley, Andrew J. (author)
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- Froguel, Philippe (author)
- Hammersmith Hospital,Centre national de la recherche scientifique (CNRS)
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- Carlsson, Lena M S, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Uhlén, Mathias (author)
- KTH,Proteomik,Albanova VinnExcellence Center for Protein Technology, ProNova,Alba Nova Universitetscentrum,AlbaNova University Center. Stockholm Center for Physics, Astronomy and Biotechnology
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- Nielsen, Jens B, 1962 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2013-03-19
- 2013
- English.
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In: Molecular Systems Biology. - : EMBO. - 1744-4292 .- 1744-4292. ; 9, s. 649-
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Abstract
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- We evaluated the presence/absence of proteins encoded by 14 077 genes in adipocytes obtained from different tissue samples using immunohistochemistry. By combining this with previously published adipocyte-specific proteome data, we identified proteins associated with 7340 genes in human adipocytes. This information was used to reconstruct a comprehensive and functional genome-scale metabolic model of adipocyte metabolism. The resulting metabolic model, iAdipocytes1809, enables mechanistic insights into adipocyte metabolism on a genome-wide level, and can serve as a scaffold for integration of omics data to understand the genotype-phenotype relationship in obese subjects. By integrating human transcriptome and fluxome data, we found an increase in the metabolic activity around androsterone, ganglioside GM2 and degradation products of heparan sulfate and keratan sulfate, and a decrease in mitochondrial metabolic activities in obese subjects compared with lean subjects. Our study hereby shows a path to identify new therapeutic targets for treating obesity through combination of high throughput patient data and metabolic modeling.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- adipocyte
- flux balance analysis
- genome-scale metabolic model
- obesity
- proteome
- adipocyte
- flux balance analysis
- genome-scale metabolic model
- obesity
- proteome
Publication and Content Type
- ref (subject category)
- art (subject category)
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Mardinoglu, Adil ...
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Ågren, Rasmus, 1 ...
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Kampf, Caroline
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Asplund, Anna
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Nookaew, Intawat ...
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Jacobson, Peter, ...
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Walley, Andrew J ...
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Froguel, Philipp ...
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Carlsson, Lena M ...
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Uhlén, Mathias
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Nielsen, Jens B, ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
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Molecular System ...
- By the university
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Uppsala University
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Royal Institute of Technology
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Chalmers University of Technology
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University of Gothenburg