onr:"swepub:oai:DiVA.org:uu-200457"
Search: onr:"swepub:oai:DiVA.org:uu-200457" > Bioassay-guided Sup...
- 1 of 1
- Previous record
- Next record
- To hitlist
Bioassay-guided Supercritical Fluid Extraction of Cyclooxygenase-2 Inhibiting Substances in Plantago major L.
- Stenholm, A. (author)
-
- Göransson, Ulf (author)
- Uppsala universitet,Avdelningen för farmakognosi
-
- Bohlin, Lars (author)
- Uppsala universitet,Avdelningen för farmakognosi
- (creator_code:org_t)
- 2012-09-19
- 2013
- English.
- In: Phytochemical Analysis. - : Wiley. - 0958-0344 .- 1099-1565. ; 24:2, s. 176-183
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Introduction - Selective extraction of plant materials is advantageous for obtaining extracts enriched with desired constituents, thereby reducing the need for subsequent chromatography purification. Such compounds include three cyclooxygenase-2 (COX-2) inhibitory substances in Plantago major L. targeted in this investigation: alpha-linolenic acid (alpha-LNA) (18:3 omega-3) and the triterpenic acids ursolic acid and oleanolic acid. Objective - To investigate the scope for tuning the selectivity of supercritical fluid extraction (SFE) using bioassay guidance, and Soxhlet extraction with dichloromethane as solvent as a reference technique, to optimise yields of these substances. Method - Extraction parameters were varied to optimise extracts' COX-2/COX-1 inhibitory effect ratios. The crude extracts were purified initially using a solid phase extraction (SPE) clean-up procedure and the target compounds were identified with GC-MS, LC-ESI-MS and LC-ESI-MS2 using GC-FID for quantification. Results - alpha-LNA was preferentially extracted in dynamic mode using unmodified carbon dioxide at 40 degrees C and 172 bar, at a 0.04% (w/w) yield with a COX-2/COX-1 inhibitory effect ratio of 1.5. Ursolic and oleanolic acids were dynamically extracted at 0.25% and 0.06% yields, respectively, with no traces of (alpha-LNA) and a COX-2/COX-1-inhibitory effect ratio of 1.1 using 10% (v/v) ethanol as polar modifier at 75 degrees C and 483 bar. The Soxhlet extracts had ursolic acid, oleanolic acid and alpha LNA yields up to 1.36%, 0.34% and 0.15%, respectively, with a COX-2/COX-1 inhibitory effect ratio of 1.2. Conclusion - The target substances can be extracted selectively by bioassay guided optimisation of SFE conditions.
Keyword
- In vitro enzyme assay
- Soxhlet
- supercritical fluid extraction (SFE)
- cyclooxygenase-2
- Plantago major L.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Phytochemical Analysis (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Stenholm, A.
- Göransson, Ulf
- Bohlin, Lars
- Articles in the publication
- Phytochemical An ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
