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VE-PTP regulates VE...
VE-PTP regulates VEGFR2 activity in stalk cells to establish endothelial cell polarity and lumen formation
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- Hayashi, Makoto (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Majumdar, Arindam (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Li, Xiujuan (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Adler, Jeremy (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Sun, Zuyue (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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Vertuani, Simona (author)
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Hellberg, Carina (author)
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- Mellberg, Sofie (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Koch, Sina (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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- Dimberg, Anna (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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Koh, Gou Young (author)
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Dejana, Elisabetta (author)
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Belting, Heinz-Georg (author)
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Affolter, Markus (author)
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Thurston, Gavin (author)
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- Holmgren, Lars (author)
- Karolinska Institutet
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Vestweber, Dietmar (author)
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- Claesson-Welsh, Lena (author)
- Uppsala universitet,Cancer och vaskulärbiologi
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(creator_code:org_t)
- 2013-04-09
- 2013
- English.
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 4, s. 1672-
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Vascular endothelial growth factor (VEGF) guides the path of new vessel sprouts by inducing VEGF receptor-2 activity in the sprout tip. In the stalk cells of the sprout, VEGF receptor-2 activity is downregulated. Here, we show that VEGF receptor-2 in stalk cells is dephosphorylated by the endothelium-specific vascular endothelial-phosphotyrosine phosphatase (VE-PTP). VE-PTP acts on VEGF receptor-2 located in endothelial junctions indirectly, via the Angiopoietin-1 receptor Tie2. VE-PTP inactivation in mouse embryoid bodies leads to excess VEGF receptor-2 activity in stalk cells, increased tyrosine phosphorylation of VE-cadherin and loss of cell polarity and lumen formation. Vessels in ve-ptp(-/-) teratomas also show increased VEGF receptor-2 activity and loss of endothelial polarization. Moreover, the zebrafish VE-PTP orthologue ptp-rb is essential for polarization and lumen formation in intersomitic vessels. We conclude that the role of Tie2 in maintenance of vascular quiescence involves VE-PTP-dependent dephosphorylation of VEGF receptor-2, and that VEGF receptor-2 activity regulates VE-cadherin tyrosine phosphorylation, endothelial cell polarity and lumen formation.
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- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Hayashi, Makoto
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Majumdar, Arinda ...
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Li, Xiujuan
-
Adler, Jeremy
-
Sun, Zuyue
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Vertuani, Simona
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show more...
-
Hellberg, Carina
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Mellberg, Sofie
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Koch, Sina
-
Dimberg, Anna
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Koh, Gou Young
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Dejana, Elisabet ...
-
Belting, Heinz-G ...
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Affolter, Markus
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Thurston, Gavin
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Holmgren, Lars
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Vestweber, Dietm ...
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Claesson-Welsh, ...
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show less...
- Articles in the publication
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Nature Communica ...
- By the university
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Uppsala University
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Karolinska Institutet