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Molecular character...
Molecular characterization of neoplastic and normal "sister" lymphoblastoid B-cell lines from chronic lymphocytic leukemia
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- Myhrinder, Anna Lanemo, 1975- (author)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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- Hellqvist, Eva (author)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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Bergh, Ann-Charlotte (author)
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- Jansson, Mattias (author)
- Uppsala universitet,Hematologi och immunologi,Department of Genetics and Pathology, Uppsala University, SE-781 85 Uppsala, Sweden
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- Nilsson, Kenneth (author)
- Uppsala universitet,Hematologi och immunologi,Department of Genetics and Pathology, Uppsala University, SE-781 85 Uppsala, Sweden
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- Hultman, Per (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Molekylär och immunologisk patologi,Klinisk patologi och klinisk genetik
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- Jonasson, Jon (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Molekylär och immunologisk patologi,Klinisk patologi och klinisk genetik
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Buhl, Anne Mette (author)
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Pedersen, Lone Bredo (author)
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Jurlander, Jesper (author)
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- Klein, Eva (author)
- Karolinska Institutet
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Weit, Nicole (author)
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Herling, Marco (author)
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- Rosenquist, Richard (author)
- Uppsala universitet,Hematologi och immunologi,Department of Genetics and Pathology, Uppsala University, SE-781 85 Uppsala, Sweden
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- Rosén, Anders (author)
- Linköpings universitet,Hälsouniversitetet,Cellbiologi
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(creator_code:org_t)
- 2013-02-25
- 2013
- English.
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In: Leukemia and Lymphoma. - : Informa UK Limited. - 1042-8194 .- 1029-2403. ; 54:8, s. 1769-1779
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https://doi.org/10.3...
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Abstract
Subject headings
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- Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger receptors. Integration of signals from BCRs with multiple surface membrane receptors determines whether the cells will be proliferating, anergic or apoptotic. To better understand the role of antigen in leukemogenesis, CLL cell lines producing monoclonal antibodies (mAbs) will facilitate structural analysis of antigens and supply DNA for genetic studies. We present here a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 17). Authenticity and verification studies of CLL-patient origin were done by IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA/short tandem repeat (STR) fingerprinting. Innate B-cell features, i.e. natural Ab production and CD5 receptors, were present in most CLL cell lines, but in none of the normal LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference representing a renewable source of authentic Abs and DNA.
Keyword
- Chronic lymphocytic leukemia (CLL)
- lymphoblastoid cell line (LCL)
- IGHV sequence
- cell-of-origin
- natural antibodies
- antigen structure
- CD5+innate B-cells
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Myhrinder, Anna ...
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Hellqvist, Eva
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Bergh, Ann-Charl ...
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Jansson, Mattias
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Nilsson, Kenneth
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Hultman, Per
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show more...
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Jonasson, Jon
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Buhl, Anne Mette
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Pedersen, Lone B ...
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Jurlander, Jespe ...
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Klein, Eva
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Weit, Nicole
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Herling, Marco
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Rosenquist, Rich ...
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Rosén, Anders
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show less...
- Articles in the publication
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Leukemia and Lym ...
- By the university
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Uppsala University
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Linköping University
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Karolinska Institutet