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Novel readout metho...
Novel readout method for molecular diagnostic assays based on optical measurements of magnetic nanobead dynamics
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- Donolato, Marco (author)
- Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech
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- Antunes, Paula (author)
- Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech
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- Stjernberg Bejhed, Rebecca (author)
- Uppsala universitet,Fasta tillståndets fysik
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- Zardán Gómez de la Torre, Teresa (author)
- Uppsala universitet,Nanoteknologi och funktionella material
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- W. Østerberg, Frederik (author)
- Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech
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- Strömberg, Mattias (author)
- Uppsala universitet,Fasta tillståndets fysik
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- Nilsson, Mats (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University
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- Strömme, Maria, 1970- (author)
- Uppsala universitet,Nanoteknologi och funktionella material
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- Svedlindh, Peter (author)
- Uppsala universitet,Fasta tillståndets fysik
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- F. Hansen, Mikkel (author)
- Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech
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- Vavassori, Paolo (author)
- CIC nanoGUNE Consolider, San Sebastian, Spain
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(creator_code:org_t)
- 2015-01-09
- 2015
- English.
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 87:3, s. 1622-1629
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- We demonstrate detection of DNA coils formed from a Vibrio cholerae DNA target at picomolar concentrations using a novel optomagnetic approach exploiting the dynamic behavior and optical anisotropy of magnetic nanobead (MNB) assemblies. We establish that the complex second harmonic optical transmission spectra of MNB suspensions measured upon application of a weak uniaxial AC magnetic field correlate well with the rotation dynamics of the individual MNBs. Adding a target analyte to the solution leads to the formation of permanent MNB clusters, namely, to the suppression of the dynamic MNB behavior. We prove that the optical transmission spectra are highly sensitive to the formation of permanent MNB clusters and, thereby to the target analyte concentration. As a specific clinically relevant diagnostic case, we detect DNA coils formed via padlock probe recognition and isothermal rolling circle amplification and benchmark against a commercial equipment. The results demonstrate the fast optomagnetic readout of rolling circle products from bacterial DNA utilizing the dynamic properties of MNBs in a miniaturized and low-cost platform requiring only a transparent window in the chip.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Engineering Science with specialization in Nanotechnology and Functional Materials
- Teknisk fysik med inriktning mot nanoteknologi och funktionella material
- Engineering Science with specialization in Solid State Physics
- Teknisk fysik med inriktning mot fasta tillståndets fysik
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Donolato, Marco
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Antunes, Paula
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Stjernberg Bejhe ...
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Zardán Gómez de ...
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W. Østerberg, Fr ...
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Strömberg, Matti ...
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show more...
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Nilsson, Mats
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Strömme, Maria, ...
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Svedlindh, Peter
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F. Hansen, Mikke ...
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Vavassori, Paolo
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Analytical Chemi ...
- By the university
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Uppsala University
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Stockholm University