Search: onr:"swepub:oai:DiVA.org:uu-213733" >
All-trans retinoic ...
All-trans retinoic acid prevents epidural fibrosis through NF-kappa B signaling pathway in post-laminectomy rats
-
Zhang, Chao (author)
-
Kong, Xiaohong (author)
-
Ning, Guangzhi (author)
-
show more...
-
Liang, Zhipin (author)
-
Qu, Tongjun (author)
-
Chen, Feiran (author)
-
Cao, Daigui (author)
-
Wang, Tianyi (author)
-
- Sharma, Hari Shanker (author)
- Uppsala universitet,Anestesiologi och intensivvård
-
Feng, Shiqing (author)
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- English.
-
In: Neuropharmacology. - : Elsevier BV. - 0028-3908 .- 1873-7064. ; 79C, s. 275-281
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Laminectomy is a widely accepted treatment for lumbar disorders, and epidural fibrosis (EF) is a common complication. EF is thought to cause post-operative pain recurrence after laminectomy or discectomy. All-trans retinoic acid (ATRA) has shown anti-fibrotic, anti-inflammatory, and anti-proliferative functions. The object of this study was to investigate the effects of ATRA on the prevention of EF in post-laminectomy rats. In vitro, the anti-fibrotic effect of ATRA was demonstrated with cultured fibroblasts count, which comprised of those that were cultured with/without ATRA. In vivo, rats underwent laminectomy at the L1-L2 levels. We first demonstrated the beneficial effects using 0.05% ATRA compared to vehicle (control group). We found that a higher concentration of ATRA (0.1%) achieved dose-dependent results. Hydroxyproline content, Rydell score, vimentin-positive cell density, fibroblast density, inflammatory cell density and inflammatory factor expression levels all suggested better outcomes in the 0.1% ATRA rats compared to the other three groups. Presumably, these effects involved ATRA's ability to suppress transforming growth factor (TGF-β1) and interleukin (IL)-6 which was confirmed with reverse-transcriptase polymerase chain reaction (RT-PCR). Finally we demonstrated that ATRA down-regulated nuclear factor (NF)-κB by immunohistochemistry and western blotting for p65 and inhibition of κB (IκBα), respectively. Our findings indicate that topical application of ATRA can inhibit fibroblast proliferation, decrease TGF-β1 and IL-6 expression level, and prevent epidural scar adhesion in rats. The highest concentration employed in this study (0.1%) was the most effective. ATRA suppressed EF through down-regulating NF-κB signaling, whose specific mechanism is suppression of IκB phosphorylation and proteolytic degradation.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Zhang, Chao
-
Kong, Xiaohong
-
Ning, Guangzhi
-
Liang, Zhipin
-
Qu, Tongjun
-
Chen, Feiran
-
show more...
-
Cao, Daigui
-
Wang, Tianyi
-
Sharma, Hari Sha ...
-
Feng, Shiqing
-
show less...
- Articles in the publication
-
Neuropharmacolog ...
- By the university
-
Uppsala University