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Healthy human CSF p...
Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks
- Article/chapterEnglish2013
Publisher, publication year, extent ...
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2013-05-13
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The Company of Biologists,2013
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-216183
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-216183URI
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https://doi.org/10.1242/bio.20134648DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option in regenerative medicine have been well recognized. These cells also offer an interesting tool for in vitro models of neuronal networks to be used for drug screening and neurotoxicological studies and for patient/disease specific in vitro models. Here, as aiming to develop a reductionistic in vitro human neuronal network model, we tested whether human embryonic stem cell (hESC)-derived neural cells could be cultured in human cerebrospinal fluid (CSF) in order to better mimic the in vivo conditions. Our results showed that CSF altered the differentiation of hESC-derived neural cells towards glial cells at the expense of neuronal differentiation. The proliferation rate was reduced in CSF cultures. However, even though the use of CSF as the culture medium altered the glial vs. neuronal differentiation rate, the pre-existing spontaneous activity of the neuronal networks persisted throughout the study. These results suggest that it is possible to develop fully human cell and culture-based environments that can further be modified for various in vitro modeling purposes.
Added entries (persons, corporate bodies, meetings, titles ...)
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Äänismaa, Riikka
(author)
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Tenhunen, JyrkiUppsala universitet,Anestesiologi och intensivvård,Critical Care Medicine Research Group, Department of Intensive Care Unit, Tampere University Hospital, FI-33521 Tampere, Finland(Swepub:uu)jyrte348
(author)
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Hagman, Sanna
(author)
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Ylä-Outinen, Laura
(author)
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Aho, Antti
(author)
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Yli-Hankala, Arvi
(author)
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Bendel, Stepani
(author)
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Skottman, Heli
(author)
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Narkilahti, Susanna
(author)
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Uppsala universitetAnestesiologi och intensivvård
(creator_code:org_t)
Related titles
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In:Biology open: The Company of Biologists2:6, s. 605-6122046-6390
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Kiiski, Heikki
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Äänismaa, Riikka
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Tenhunen, Jyrki
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Hagman, Sanna
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Ylä-Outinen, Lau ...
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Aho, Antti
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Yli-Hankala, Arv ...
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Bendel, Stepani
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Skottman, Heli
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Narkilahti, Susa ...
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Biology open
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Uppsala University