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Serial Femtosecond ...
Serial Femtosecond Crystallography of G Protein-Coupled Receptors
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Liu, Wei (author)
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Wacker, Daniel (author)
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Gati, Cornelius (author)
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Han, Gye Won (author)
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James, Daniel (author)
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Wang, Dingjie (author)
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Nelson, Garrett (author)
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Weierstall, Uwe (author)
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Katritch, Vsevolod (author)
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Barty, Anton (author)
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Zatsepin, Nadia A. (author)
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Li, Dianfan (author)
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Messerschmidt, Marc (author)
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Boutet, Sebastien (author)
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Williams, Garth J. (author)
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Koglin, Jason E. (author)
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- Seibert, M. Marvin (author)
- Uppsala universitet,Molekylär biofysik
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Wang, Chong (author)
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Shah, Syed T. A. (author)
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Basu, Shibom (author)
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Fromme, Raimund (author)
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Kupitz, Christopher (author)
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Rendek, Kimberley N. (author)
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Grotjohann, Ingo (author)
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Fromme, Petra (author)
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Kirian, Richard A. (author)
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Beyerlein, Kenneth R. (author)
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White, Thomas A. (author)
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Chapman, Henry N. (author)
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Caffrey, Martin (author)
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Spence, John C. H. (author)
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Stevens, Raymond C. (author)
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Cherezov, Vadim (author)
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(creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2013
- 2013
- English.
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 342:6165, s. 1521-1524
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https://bib-pubdb1.d...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) with individual 50-femtosecond-duration x-ray pulses to minimize radiation damage and obtained a high-resolution room-temperature structure of a human serotonin receptor using sub-10-micrometer microcrystals grown in a membrane mimetic matrix known as lipidic cubic phase. Compared with the structure solved by using traditional microcrystallography from cryo-cooled crystals of about two orders of magnitude larger volume, the room-temperature XFEL structure displays a distinct distribution of thermal motions and conformations of residues that likely more accurately represent the receptor structure and dynamics in a cellular environment.
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- art (subject category)
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Science
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- By the author/editor
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Liu, Wei
-
Wacker, Daniel
-
Gati, Cornelius
-
Han, Gye Won
-
James, Daniel
-
Wang, Dingjie
-
show more...
-
Nelson, Garrett
-
Weierstall, Uwe
-
Katritch, Vsevol ...
-
Barty, Anton
-
Zatsepin, Nadia ...
-
Li, Dianfan
-
Messerschmidt, M ...
-
Boutet, Sebastie ...
-
Williams, Garth ...
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Koglin, Jason E.
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Seibert, M. Marv ...
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Wang, Chong
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Shah, Syed T. A.
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Basu, Shibom
-
Fromme, Raimund
-
Kupitz, Christop ...
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Rendek, Kimberle ...
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Grotjohann, Ingo
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Fromme, Petra
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Kirian, Richard ...
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Beyerlein, Kenne ...
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White, Thomas A.
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Chapman, Henry N ...
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Caffrey, Martin
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Spence, John C. ...
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Stevens, Raymond ...
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Cherezov, Vadim
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show less...
- Articles in the publication
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Science
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Uppsala University