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  • Liu, Wei (author)

Serial Femtosecond Crystallography of G Protein-Coupled Receptors

  • Article/chapterEnglish2013

Publisher, publication year, extent ...

  • American Association for the Advancement of Science (AAAS),2013
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-217630
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-217630URI
  • https://doi.org/10.1126/science.1244142DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) with individual 50-femtosecond-duration x-ray pulses to minimize radiation damage and obtained a high-resolution room-temperature structure of a human serotonin receptor using sub-10-micrometer microcrystals grown in a membrane mimetic matrix known as lipidic cubic phase. Compared with the structure solved by using traditional microcrystallography from cryo-cooled crystals of about two orders of magnitude larger volume, the room-temperature XFEL structure displays a distinct distribution of thermal motions and conformations of residues that likely more accurately represent the receptor structure and dynamics in a cellular environment.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Wacker, Daniel (author)
  • Gati, Cornelius (author)
  • Han, Gye Won (author)
  • James, Daniel (author)
  • Wang, Dingjie (author)
  • Nelson, Garrett (author)
  • Weierstall, Uwe (author)
  • Katritch, Vsevolod (author)
  • Barty, Anton (author)
  • Zatsepin, Nadia A. (author)
  • Li, Dianfan (author)
  • Messerschmidt, Marc (author)
  • Boutet, Sebastien (author)
  • Williams, Garth J. (author)
  • Koglin, Jason E. (author)
  • Seibert, M. MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255 (author)
  • Wang, Chong (author)
  • Shah, Syed T. A. (author)
  • Basu, Shibom (author)
  • Fromme, Raimund (author)
  • Kupitz, Christopher (author)
  • Rendek, Kimberley N. (author)
  • Grotjohann, Ingo (author)
  • Fromme, Petra (author)
  • Kirian, Richard A. (author)
  • Beyerlein, Kenneth R. (author)
  • White, Thomas A. (author)
  • Chapman, Henry N. (author)
  • Caffrey, Martin (author)
  • Spence, John C. H. (author)
  • Stevens, Raymond C. (author)
  • Cherezov, Vadim (author)
  • Uppsala universitetMolekylär biofysik (creator_code:org_t)

Related titles

  • In:Science: American Association for the Advancement of Science (AAAS)342:6165, s. 1521-15240036-80751095-9203

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