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Serial Femtosecond ...
Serial Femtosecond Crystallography of G Protein-Coupled Receptors
- Article/chapterEnglish2013
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American Association for the Advancement of Science (AAAS),2013
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LIBRIS-ID:oai:DiVA.org:uu-217630
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-217630URI
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https://doi.org/10.1126/science.1244142DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) with individual 50-femtosecond-duration x-ray pulses to minimize radiation damage and obtained a high-resolution room-temperature structure of a human serotonin receptor using sub-10-micrometer microcrystals grown in a membrane mimetic matrix known as lipidic cubic phase. Compared with the structure solved by using traditional microcrystallography from cryo-cooled crystals of about two orders of magnitude larger volume, the room-temperature XFEL structure displays a distinct distribution of thermal motions and conformations of residues that likely more accurately represent the receptor structure and dynamics in a cellular environment.
Added entries (persons, corporate bodies, meetings, titles ...)
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Wacker, Daniel
(author)
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Gati, Cornelius
(author)
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Han, Gye Won
(author)
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James, Daniel
(author)
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Wang, Dingjie
(author)
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Nelson, Garrett
(author)
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Weierstall, Uwe
(author)
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Katritch, Vsevolod
(author)
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Barty, Anton
(author)
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Zatsepin, Nadia A.
(author)
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Li, Dianfan
(author)
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Messerschmidt, Marc
(author)
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Boutet, Sebastien
(author)
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Williams, Garth J.
(author)
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Koglin, Jason E.
(author)
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Seibert, M. MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255
(author)
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Wang, Chong
(author)
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Shah, Syed T. A.
(author)
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Basu, Shibom
(author)
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Fromme, Raimund
(author)
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Kupitz, Christopher
(author)
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Rendek, Kimberley N.
(author)
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Grotjohann, Ingo
(author)
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Fromme, Petra
(author)
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Kirian, Richard A.
(author)
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Beyerlein, Kenneth R.
(author)
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White, Thomas A.
(author)
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Chapman, Henry N.
(author)
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Caffrey, Martin
(author)
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Spence, John C. H.
(author)
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Stevens, Raymond C.
(author)
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Cherezov, Vadim
(author)
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Uppsala universitetMolekylär biofysik
(creator_code:org_t)
Related titles
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In:Science: American Association for the Advancement of Science (AAAS)342:6165, s. 1521-15240036-80751095-9203
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Liu, Wei
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Wacker, Daniel
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Gati, Cornelius
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Han, Gye Won
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James, Daniel
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Wang, Dingjie
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Nelson, Garrett
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Weierstall, Uwe
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Katritch, Vsevol ...
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Barty, Anton
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Zatsepin, Nadia ...
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Li, Dianfan
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Messerschmidt, M ...
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Boutet, Sebastie ...
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Williams, Garth ...
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Koglin, Jason E.
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Seibert, M. Marv ...
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Wang, Chong
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Shah, Syed T. A.
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Basu, Shibom
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Fromme, Raimund
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Kupitz, Christop ...
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Rendek, Kimberle ...
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Grotjohann, Ingo
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Fromme, Petra
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Kirian, Richard ...
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Beyerlein, Kenne ...
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White, Thomas A.
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Chapman, Henry N ...
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Caffrey, Martin
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Spence, John C. ...
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Stevens, Raymond ...
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Cherezov, Vadim
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