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Quantitative Imagin...
Quantitative Imaging of Serotonergic Biosynthesis and Degradation in the Endocrine Pancreas
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- Eriksson, Olof (author)
- Uppsala universitet,Plattformen för preklinisk PET
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- Selvaraju, Ram K (author)
- Uppsala universitet,Plattformen för preklinisk PET
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- Johansson, Lars (author)
- Uppsala universitet,Enheten för radiologi
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- Eriksson, Jan W (author)
- Uppsala universitet,Klinisk diabetologi och metabolism
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- Sundin, Anders (author)
- Uppsala universitet,Enheten för radiologi
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- Antoni, Gunnar (author)
- Uppsala universitet,Enheten för onkologi
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- Sörensen, Jens (author)
- Uppsala universitet,Enheten för nuklearmedicin och PET
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- Eriksson, Barbro (author)
- Uppsala universitet,Endokrin tumörbiologi
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- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
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(creator_code:org_t)
- 2014-02-13
- 2014
- English.
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In: Journal of Nuclear Medicine. - : Society of Nuclear Medicine. - 0161-5505 .- 1535-5667 .- 2159-662X. ; 55:3, s. 460-465
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http://jnm.snmjourna...
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https://urn.kb.se/re...
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https://doi.org/10.2...
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Abstract
Subject headings
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- Serotonergic biosynthesis in the endocrine pancreas, of which the islets of Langerhans is the major constituent, has been implicated in insulin release and β cell proliferation. In this study, we investigated the feasibility of quantitative noninvasive imaging of the serotonergic metabolism in the pancreas using the PET tracer (11)C-5-hydroxy-l-tryptophan ((11)C-5-HTP).METHODS: Uptake of (11)C-5-HTP, and its specificity for key enzymes in the serotonergic metabolic pathway, was assessed in vitro (INS-1 and PANC1 cells and human islet and exocrine preparations) and in vivo (nonhuman primates and healthy and diabetic rats).RESULTS: In vitro tracer uptake in endocrine cells (INS-1 and human islets), but not PANC1 and exocrine cells, was mediated specifically by intracellular conversion into serotonin. Pancreatic uptake of (11)C-5-HTP in nonhuman primates was markedly decreased by inhibition of the enzyme dopa decarboxylase, which converts (11)C-5-HTP to (11)C-serotonin and increased after inhibition of monoamine oxidase-A, the main enzyme responsible for serotonin degradation. Uptake in the rat pancreas was similarly modulated by inhibition of monoamine oxidase-A and was reduced in animals with induced diabetes.CONCLUSION: The PET tracer (11)C-5-HTP can be used for quantitative imaging of the serotonergic system in the endocrine pancreas.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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