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  • Weierstall, Uwe (author)

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

  • Article/chapterEnglish2014

Publisher, publication year, extent ...

  • 2014-02-14
  • Springer Science and Business Media LLC,2014
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-223577
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-223577URI
  • https://doi.org/10.1038/ncomms4309DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mu m-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Added entries (persons, corporate bodies, meetings, titles ...)

  • James, Daniel (author)
  • Wang, Chong (author)
  • White, Thomas A. (author)
  • Wang, Dingjie (author)
  • Liu, Wei (author)
  • Spence, John C. H. (author)
  • Doak, R. Bruce (author)
  • Nelson, Garrett (author)
  • Fromme, Petra (author)
  • Fromme, Raimund (author)
  • Grotjohann, Ingo (author)
  • Kupitz, Christopher (author)
  • Zatsepin, Nadia A. (author)
  • Liu, Haiguang (author)
  • Basu, Shibom (author)
  • Wacker, Daniel (author)
  • Han, Gye Won (author)
  • Katritch, Vsevolod (author)
  • Boutet, Sebastien (author)
  • Messerschmidt, Marc (author)
  • Williams, Garth J. (author)
  • Koglin, Jason E. (author)
  • Seibert, Marvin M.Uppsala universitet,Molekylär biofysik(Swepub:uu)masei255 (author)
  • Klinker, Markus (author)
  • Gati, Cornelius (author)
  • Shoeman, Robert L. (author)
  • Barty, Anton (author)
  • Chapman, Henry N. (author)
  • Kirian, Richard A. (author)
  • Beyerlein, Kenneth R. (author)
  • Stevens, Raymond C. (author)
  • Li, Dianfan (author)
  • Shah, Syed T. A. (author)
  • Howe, Nicole (author)
  • Caffrey, Martin (author)
  • Cherezov, Vadim (author)
  • Uppsala universitetMolekylär biofysik (creator_code:org_t)

Related titles

  • In:Nature Communications: Springer Science and Business Media LLC5, s. 3309-2041-1723

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