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Lipidic cubic phase...
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
- Article/chapterEnglish2014
Publisher, publication year, extent ...
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2014-02-14
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Springer Science and Business Media LLC,2014
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:uu-223577
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-223577URI
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https://doi.org/10.1038/ncomms4309DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-mu m-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Added entries (persons, corporate bodies, meetings, titles ...)
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James, Daniel
(author)
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Wang, Chong
(author)
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White, Thomas A.
(author)
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Wang, Dingjie
(author)
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Liu, Wei
(author)
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Spence, John C. H.
(author)
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Doak, R. Bruce
(author)
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Nelson, Garrett
(author)
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Fromme, Petra
(author)
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Fromme, Raimund
(author)
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Grotjohann, Ingo
(author)
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Kupitz, Christopher
(author)
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Zatsepin, Nadia A.
(author)
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Liu, Haiguang
(author)
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Basu, Shibom
(author)
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Wacker, Daniel
(author)
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Han, Gye Won
(author)
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Katritch, Vsevolod
(author)
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Boutet, Sebastien
(author)
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Messerschmidt, Marc
(author)
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Williams, Garth J.
(author)
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Koglin, Jason E.
(author)
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Seibert, Marvin M.Uppsala universitet,Molekylär biofysik(Swepub:uu)masei255
(author)
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Klinker, Markus
(author)
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Gati, Cornelius
(author)
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Shoeman, Robert L.
(author)
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Barty, Anton
(author)
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Chapman, Henry N.
(author)
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Kirian, Richard A.
(author)
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Beyerlein, Kenneth R.
(author)
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Stevens, Raymond C.
(author)
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Li, Dianfan
(author)
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Shah, Syed T. A.
(author)
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Howe, Nicole
(author)
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Caffrey, Martin
(author)
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Cherezov, Vadim
(author)
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Uppsala universitetMolekylär biofysik
(creator_code:org_t)
Related titles
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In:Nature Communications: Springer Science and Business Media LLC5, s. 3309-2041-1723
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Weierstall, Uwe
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James, Daniel
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Wang, Chong
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White, Thomas A.
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Wang, Dingjie
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Liu, Wei
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Spence, John C. ...
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Doak, R. Bruce
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Nelson, Garrett
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Fromme, Petra
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Fromme, Raimund
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Grotjohann, Ingo
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Kupitz, Christop ...
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Zatsepin, Nadia ...
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Liu, Haiguang
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Basu, Shibom
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Wacker, Daniel
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Han, Gye Won
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Katritch, Vsevol ...
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Boutet, Sebastie ...
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Messerschmidt, M ...
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Williams, Garth ...
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Koglin, Jason E.
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Seibert, Marvin ...
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Klinker, Markus
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Gati, Cornelius
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Shoeman, Robert ...
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Barty, Anton
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Chapman, Henry N ...
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Kirian, Richard ...
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Beyerlein, Kenne ...
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Stevens, Raymond ...
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Li, Dianfan
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Shah, Syed T. A.
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Howe, Nicole
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Caffrey, Martin
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Cherezov, Vadim
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