onr:"swepub:oai:DiVA.org:uu-223787"
Search: onr:"swepub:oai:DiVA.org:uu-223787" > A visualizable chai...
- 1 of 1
- Previous record
- Next record
- To hitlist
A visualizable chain-terminating inhibitor of glycosaminoglycan biosynthesis in developing zebrafish
- Beahm, Brendan J (author)
- Dehnert, Karen W (author)
- Derr, Nicolas L (author)
- show more...
- Kuhn, Joachim (author)
- Eberhart, Johann K (author)
- Amacher, Sharon L (author)
- Bertozzi, Carolyn R (author)
- show less...
- (creator_code:org_t)
- 2014-02-19
- 2014
- English.
- In: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 53:13, s. 3347-3352
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Heparan sulfate (HS) and chondroitin sulfate (CS) glycosaminoglycans (GAG) are proteoglycan-associated polysaccharides with essential functions in animals. They have been studied extensively by genetic manipulation of biosynthetic enzymes, but chemical tools for probing GAG function are limited. HS and CS possess a conserved xylose residue that links the polysaccharide chain to a protein backbone. Here we report that, in zebrafish embryos, the peptide-proximal xylose residue can be metabolically replaced with a chain-terminating 4-azido-4-deoxyxylose (4-XylAz) residue by administration of UDP-4-azido-4-deoxyxylose (UDP-4-XylAz). UDP-4-XylAz disrupted both HS and CS biosynthesis and caused developmental abnormalities reminiscent of GAG biosynthesis and laminin mutants. The azide substituent of protein-bound 4-XylAz allowed for rapid visualization of the organismal sites of chain termination in vivo through bioorthogonal reaction with fluorescent cyclooctyne probes. UDP-4-XylAz therefore complements genetic tools for studies of GAG function in zebrafish embryogenesis.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Angewandte Chemie International Edition (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Beahm, Brendan J
- Dehnert, Karen W
- Derr, Nicolas L
- Kuhn, Joachim
- Eberhart, Johann ...
- Spillmann, Dorot ...
- show more...
- Amacher, Sharon ...
- Bertozzi, Caroly ...
- show less...
- Articles in the publication
- Angewandte Chemi ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
