The nanoparticulate Quillaja saponin KGI exerts anti-proliferative eff ects by down-regulation of cell cycle molecules in U937 and HL-60 human leukemia cells

Berenjian, Saideh (author)

Hu, Kefei (author)

Hassan, Sadia Bashir (author): Uppsala universitet,Cancerfarmakologi och beräkningsmedicin

Morein, Bror (author): Uppsala universitet,Klinisk mikrobiologi och infektionsmedicin

(creator_code:org_t)

2013-12-02
2014
English.
In: Leukemia and Lymphoma. - : Informa UK Limited. - 1042-8194 .- 1029-2403. ; 55:7, s. 1618-1624

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.3...; http://kipublication...; show less...

Abstract Subject headings

Cancer cells are characterized by uncontrolled replication involving loss of control of cyclin dependent kinases (CDKs) and cyclins, and by abolished differentiation. In this study we introduce KGI, which is a nanoparticle with a Quillaja saponin as an active molecule. By the use of RNA array analysis and confirmation at the protein level, we show that KGI affects myeloid leukemia cells (in particular, the U937 monoblast cancer cell) by the following mechanisms: (A) ceasing cell replication via proteasome degradation, (B) down-regulation of key molecules at check points between G1/S and G2/M phases, (C) reduction of thymidine kinase activity, followed by (D) exit to differentiation and production of interleukin-8 (IL-8), eventually leading to apoptosis. Leukemia cell lines (U937 and HL-60 cells) were exposed to KGI for 8 h, after which the drug was removed. The cancer cells did not revert to replication over the following 10 days. Thus our findings suggest that the nanoparticle KGI inhibits proliferation and promotes differentiation in leukemic cells by interfering with the cell cycle process.

By the author/editor: Berenjian, Saide ...; Hu, Kefei; Abedi-Valugerdi, ...; Hassan, Moustaph ...; Hassan, Sadia Ba ...; Morein, Bror

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Hematology

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