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Amplification of 2p...
Amplification of 2p as a Genomic Marker for Transformation in Lymphoma
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- Kwiecinska, Anna (author)
- Karolinska Institutet
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Ichimura, Koichi (author)
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- Berglund, Mattias (author)
- Karolinska Institutet,Uppsala universitet,Enheten för onkologi
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- Dinets, Andrii (author)
- Karolinska Institutet
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- Sulaiman, Luqman (author)
- Karolinska Institutet
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Collins, V. Peter (author)
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- Larsson, Catharina (author)
- Karolinska Institutet
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- Porwit, Anna (author)
- Karolinska Institutet
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- Lagercrantz, Svetlana Bajalica (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2014-05-15
- 2014
- English.
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In: Genes, Chromosomes and Cancer. - : Wiley. - 1045-2257 .- 1098-2264. ; 53:9, s. 750-768
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Abstract
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- To outline further genetic mechanisms of transformation from follicular lymphoma (FL) to diffuse large B-cell lymphoma (DLBCL), we have performed whole genome array-CGH in 81 tumors from 60 patients [29 de novo DLBCL (dnDLBCL), 31 transformed DLBCL (tDLBCL), and 21 antecedent FL]. In 15 patients, paired tumor samples (primary FL and a subsequent tDLBCL) were available, among which three possessed more than two subsequent tumors, allowing us to follow specific genetic alterations acquired before, during, and after the transformation. Gain of 2p15-16.1 encompassing, among others, the REL, BCL11A, USP34, COMMD1, and OTX1 genes was found to be more common in the tDLBCL compared with dnDLBCL (P < 0.001). Furthermore, a high-level amplification of 2p15-16.1 was also detected in the FL stage prior to transformation, indicating its importance during the transformation event. Quantitative real-time PCR showed a higher level of amplification of REL, USP34, and COMMD1 (all involved in the NF kappa B-pathway) compared with BCL11A, which indicates that the altered genes disrupting the NF kappa B pathway may be the driver genes of transformation rather than the previously suggested BCL11A. Moreover, a 17q21.33 amplification was exclusively found in tDLBCL, never in FL (P < 0.04) or dnDLBCL, indicating an upregulation of genes of importance during the later phase of transformation. Taken together, our study demonstrates potential genomic markers for disease progression to clinically more aggressive forms. We also confirm the importance of the TP53-, CDKN2A-, and NF kappa B-pathways for the transformation from FL to DLBCL.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kwiecinska, Anna
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Ichimura, Koichi
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Berglund, Mattia ...
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Dinets, Andrii
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Sulaiman, Luqman
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Collins, V. Pete ...
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show more...
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Larsson, Cathari ...
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Porwit, Anna
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Lagercrantz, Sve ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Genes, Chromosom ...
- By the university
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Uppsala University
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Karolinska Institutet