Effect of Glyceryl Trinitrate on Staphylococcus aureus Growth and Leukocyte Activation during Simulated Extracorporeal Circulation

Background Previously, nitric oxide has been shown to possess antimicrobial effects. In this study, we aim to test the effect of glyceryl trinitrate (GTN) on Staphylococcus aureus growth during simulated extracorporeal circulation (SECC) and also to examine the effect of S. aureus, alone and in combination with GTN, on activation markers of the innate immune system during SECC. Methods In an in vitro system of SECC, we measured GTN-induced changes in markers of leukocyte activation in whole blood caused by S. aureus infestation, as well as the effect of GTN on S. aureus growth. Results GTN had no effect on S. aureus growth after 240 minutes SECC. Staphylococcus aureus reduced the expression of granulocyte Fc gamma-receptor CD32 but stimulated the expression of monocyte CD32. Staphylococcus aureus stimulated expression of some leukocyte adhesion key proteins, activation marker CD66b, lipopolysaccharide-receptor CD14, and C3b-receptor CD35. Staphylococcus aureus and GTN addition induced significant increases in monocyte CD63 (lysosomal granule protein) levels. Conclusion GTN does not affect S. aureus growth during SECC and has no effect on SECC-induced leukocyte activation.

Tano, EvaUppsala universitet,Klinisk bakteriologi(Swepub:uu)evata102 (author)
Hakansson, Lena Douhan (author)
Tran, Phan-KietUppsala universitet,Institutionen för kirurgiska vetenskaper (author)
Knutson, FolkeUppsala universitet,Klinisk immunologi(Swepub:uu)folkknut (author)
Malinski, Tadeusz (author)
Borowiec, JanUppsala universitet,Thoraxkirurgi(Swepub:uu)janborow (author)
Uppsala universitetThoraxkirurgi (creator_code:org_t)

In:The thoracic and cardiovascular surgeon: Georg Thieme Verlag KG62:5, s. 402-4080171-64251439-1902

By the author/editor: Melki, Vilyam; Tano, Eva; Hakansson, Lena ...; Tran, Phan-Kiet; Knutson, Folke; Malinski, Tadeus ...; show more...; Borowiec, Jan; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Surgery

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cardiac and Card ...

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