Fixed-target protein serial microcrystallography with an x-ray free electron laser.

We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (~2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of ~38%, indicating that a complete data set could be acquired in about one 12-hour LCLS shift using the setup described here, or in even less time using hardware optimized for fixed target SFX. This demonstration opens the door to ultra low sample consumption SFX using the technique of diffraction-before-destruction on proteins that exist in only small quantities and/or do not produce the copious quantities of microcrystals required for flowing jet methods.

In:Scientific reports: Springer Science and Business Media LLC4, s. 6026-2045-2322

By the author/editor: Hunter, Mark S; Segelke, Brent; Messerschmidt, M ...; Williams, Garth ...; Zatsepin, Nadia ...; Barty, Anton; show more...; Benner, W Henry; Carlson, David B; Coleman, Matthew; Graf, Alexander; Hau-Riege, Stefa ...; Pardini, Tommaso; Seibert, M Marvi ...; Evans, James; Boutet, Sébastie ...; Frank, Matthias; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Structural Biolo ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.