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- Andrae, JohannaUppsala universitet,Cancer och vaskulärbiologi (author)
Characterization of Platelet-Derived Growth Factor-A Expression in Mouse Tissues Using a lacZ Knock-In Approach
- Article/chapterEnglish2014
Publisher, publication year, extent ...
- 2014-08-28
- Public Library of Science (PLoS),2014
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-233014
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-233014URI
- https://doi.org/10.1371/journal.pone.0105477DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:129667503URI
Supplementary language notes
- Language:English
- Summary in:English
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- Expression of the platelet-derived growth factor A-chain gene (Pdgfa) occurs widely in the developing mouse, where it is mainly localized to various epithelial and neuronal structures. Until now, in situ mRNA hybridization (ISH) has been the only reliable method to identify Pdgfa expression in tissue sections or whole mount preparations. Validated protocols for in situ detection of PDGF-A protein by immunohistochemistry is lacking. In particular, this has hampered understanding of Pdgfa expression pattern in adult tissues, where ISH is technically challenging. Here, we report a gene targeted mouse Pdgfa allele, Pdgfa(ex4COIN), which is a combined conditional knockout and reporter allele. Cre-mediated inversion of the COIN cassette inactivates Pdgfa coding while simultaneously activating a beta-galactosidase (lacZ) reporter under endogenous Pdgfa transcription control. The generated Pdgfa(ex4COIN-INV-lacZ) allele can next be used to identify cells carrying a Pdgfa null allele, as well as to map endogenous Pdgfa expression. We evaluated the Pdgfa(ex4COIN-INV-lacZ) allele as a reporter for endogenous Pdgfa expression patterns in mouse embryos and adults. We conclude that the expression pattern of Pdgfa(ex4COIN-INV-lacZ) recapitulates known expression patterns of Pdgfa. We also report on novel embryonic and adult Pdgfa expression patterns in the mouse and discuss their implications for Pdgfa physiology.
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Added entries (persons, corporate bodies, meetings, titles ...)
- Gouveia, Maria Leonor SeguardoUppsala universitet,Cancer och vaskulärbiologi(Swepub:uu)margo865 (author)
- He, LiqunUppsala universitet,Cancer och vaskulärbiologi(Swepub:uu)liqhe592 (author)
- Betsholtz, ChristerKarolinska Institutet (author)
- Uppsala universitetCancer och vaskulärbiologi (creator_code:org_t)
Related titles
- In:PLOS ONE: Public Library of Science (PLoS)9:8, s. e105477-1932-6203
Internet link
- https://uu.diva-portal.org/smash/get/diva2:753701/FULLTEXT01.pdffulltext:print
- https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0105477&type=printable
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-233014
- https://doi.org/10.1371/journal.pone.0105477
- http://kipublications.ki.se/Default.aspx?queryparsed=id:129667503
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