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Differential methyl...
Differential methylation in CN-AML preferentially targets non-CGI regions and is dictated by DNMT3A mutational status and associated with predominant hypomethylation of HOX genes
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- Qu, Ying (author)
- Karolinska Institutet
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- Lennartsson, Andreas (author)
- Karolinska Institutet
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Gaidzik, Verena I. (author)
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- Deneberg, Stefan (author)
- Karolinska Institutet
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- Karimi, Mohsen (author)
- Karolinska Institutet
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Bengtzen, Sofia (author)
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- Höglund, Martin (author)
- Uppsala universitet,Hematologi
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Bullinger, Lars (author)
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Doehner, Konstanze (author)
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- Lehmann, Soren (author)
- Karolinska Institutet,Uppsala universitet,Hematologi
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(creator_code:org_t)
- 2014-05-27
- 2014
- English.
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In: Epigenetics. - : Informa UK Limited. - 1559-2294 .- 1559-2308. ; 9:8, s. 1108-1119
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Abstract
Subject headings
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- The extent and role of aberrant DNA methylation in promoter CpG islands (CGIs) have been extensively studied in leukemia and other malignancies. Still, CGIs represent only a small fraction of the methylome. We aimed to characterize genome-wide differential methylation of cytogenetically normal AML (CN-AML) cells compared with normal CD34(+) bone marrow cells using the Illumina (R) 450K methylation array. Differential methylation in CN-AML was most prominent in genomic areas far from CGIs, in so called open sea regions. Furthermore, differential methylation was specifically found in genes encoding transcription factors (TFs), with WT1 being the most differentially methylated TF. Among genetic mutations in AML, DNMT3A mutations showed the most prominent association with the DNA methylation pattern, characterized by hypomethylation of CGIs (as compared with DNMT3A wild type cases). The differential methylation in DNMT3A mutant cells vs. wild type cells was predominantly found in HOX genes, which were hypomethylated. These results were confirmed and validated in an independent CN-AML cohort. In conclusion, we show that, in CN-AML, the most pronounced changes in DNA methylation occur in non-CGI regions and that DNMT3A mutations confer a pattern of global hypomethylation that specifically targets HOX genes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Keyword
- acute myeloid leukemia
- DNA methylation
- non-CGI region
- DNMT3A
- Homeobox gene family
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Qu, Ying
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Lennartsson, And ...
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Gaidzik, Verena ...
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Deneberg, Stefan
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Karimi, Mohsen
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Bengtzen, Sofia
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show more...
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Höglund, Martin
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Bullinger, Lars
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Doehner, Konstan ...
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Lehmann, Soren
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
- Articles in the publication
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Epigenetics
- By the university
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Uppsala University
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Karolinska Institutet