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Rapid Screening of ...
Rapid Screening of Mineralization Capacity of Biomaterials by Means of Quantification of Enzymatically Deposited Calcium Phosphate
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Nijhuis, Arnold W. G. (author)
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Takemoto, Shinji (author)
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Nejadnik, M. Reza (author)
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Li, Yubao (author)
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- Yang, Xia (author)
- Uppsala universitet,Polymerkemi
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- Ossipov, Dmitri A. (author)
- Uppsala universitet,Polymerkemi
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- Hilborn, Jöns (author)
- Uppsala universitet,Polymerkemi
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Mikos, Antonios G. (author)
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Yoshinari, Masao (author)
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Jansen, John A. (author)
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Leeuwenburgh, Sander C. G. (author)
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2014
- 2014
- English.
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In: Tissue Engineering. Part C, Methods. - : Mary Ann Liebert Inc. - 1937-3384 .- 1937-3392. ; 20:10, s. 838-850
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- The current study focused on the development of a rapid, straightforward quantification method based on the use of enzymatic decomposition of urea using urease to assess the mineralization capacity of a wide range of biomaterials for bone regeneration. Urea-containing mineralizing solutions (MSs) (containing: Na2HPO4, CaCl2, and NaCl at 37 degrees C and pH 6.0) were used in the mineralization experiments. Urease was added to these solutions to induce enzymatic decomposition of urea resulting in increased pH and deposition of calcium phosphate. By optimizing the ionic and urease concentrations in these MSs, it was shown that the proposed system could mineralize titanium substrates with six different pretreatments, as opposed to normal simulated body fluid that mineralized only two of them. It was possible to rank the mineralization capacity of these substrates by measuring the amount of calcium deposited. Furthermore, the ranking of (i) various polymeric substrates and (ii) hydrogels with and without functionalization with calcium-binding bisphosphonate groups was also possible. These results confirm that the proposed testing system has a broad applicability in the field of biomaterials due to its inherent versatility and discriminative power.
Subject headings
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Nijhuis, Arnold ...
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Takemoto, Shinji
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Nejadnik, M. Rez ...
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Li, Yubao
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Yang, Xia
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Ossipov, Dmitri ...
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show more...
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Hilborn, Jöns
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Mikos, Antonios ...
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Yoshinari, Masao
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Jansen, John A.
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Leeuwenburgh, Sa ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Materials Chemis ...
- Articles in the publication
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Tissue Engineeri ...
- By the university
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Uppsala University