Controlling Osteogenic Differentiation through Nanoporous Alumina

• Nanotopographical features are found to have significant effects on bone behavior. In the presentstudy, nanoporous aluminas with different pore sizes (20, 100 and 200 nm in diameter), were eva-luated for their osteoinductive and drug eluting properties. W20-17 marrow stromal cells wereseeded on nanoporous alumina with and without the addition of BMP-2. Although cell prolifera-tion was not affected by pore size, osteogenic differentiation was 200 nm as compared to 20 and100 nm pores induced higher alkaline phosphatase activity (ALP) and osteocalcin expression le-vels, thus indicating osteoblastic differentiation. Cell morphology revealed that cells cultured on20 nm pores adopted a rounded shape, while larger pores (200 nm) elicited an elongated morpho-logy. Furthermore, ALP expression levels were consistently higher on BMP-2 loaded nanoporousalumina surfaces compared to unloaded surfaces, indicating that not only is nanoporous aluminaosteoinductive, but also has the potential to be used as a drug eluting bone-implant coating.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Medical Engineering -- Medical Materials (hsv//eng)

Publication and Content Type

ref (subject category)

art (subject category)