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High-throughput ima...
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Hantke, Max F.Uppsala universitet,Molekylär biofysik
(author)
High-throughput imaging of heterogeneous cell organelles with an X-ray laser
- Article/chapterEnglish2014
Publisher, publication year, extent ...
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2014-11-17
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Springer Science and Business Media LLC,2014
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-237619
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-237619URI
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https://doi.org/10.1038/nphoton.2014.270DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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We overcome two of the most daunting challenges in single-particle diffractive imaging: collecting many high-quality diffraction patterns on a small amount of sample and separating components from mixed samples. We demonstrate this on carboxysomes, which are polyhedral cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min with the Linac Coherent Light Source running at 120 Hz. We separate different structures directly from the diffraction data and show that the size distribution is preserved during sample delivery. We automate phase retrieval and avoid reconstruction artefacts caused by missing modes. We attain the highest-resolution reconstructions on the smallest single biological objects imaged with an X-ray laser to date. These advances lay the foundations for accurate, high-throughput structure determination by flash-diffractive imaging and offer a means to study structure and structural heterogeneity in biology and elsewhere.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Hasse, DirkUppsala universitet,Molekylär biofysik(Swepub:uu)dirha931
(author)
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Maia, Filipe R. N. C.Uppsala universitet,Molekylär biofysik(Swepub:uu)fimai103
(author)
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Ekeberg, TomasUppsala universitet,Molekylär biofysik(Swepub:uu)tomek611
(author)
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John, KatjaUppsala universitet,Molekylär biofysik
(author)
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Svenda, MartinUppsala universitet,Molekylär biofysik(Swepub:uu)msv31659
(author)
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Loh, N. Duane
(author)
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Martin, Andrew V.
(author)
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Timneanu, NicusorUppsala universitet,Molekylär biofysik(Swepub:uu)nti19847
(author)
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Larsson, Daniel S.D.Uppsala universitet,Molekylär biofysik(Swepub:uu)dalar193
(author)
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Gijs, van der SchotUppsala universitet,Molekylär biofysik(Swepub:uu)gijva150
(author)
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Carlsson, Gunilla H.Uppsala universitet,Molekylär biofysik(Swepub:uu)gca29842
(author)
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Ingelman, MargaretaUppsala universitet,Molekylär biofysik(Swepub:uu)maing516
(author)
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Andreasson, JakobUppsala universitet,Molekylär biofysik(Swepub:uu)jakan998
(author)
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Westphal, DanielUppsala universitet,Molekylär biofysik(Swepub:uu)danwe345
(author)
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Liang, Mengning
(author)
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Stellato, Francesco
(author)
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DePonte, Daniel P.
(author)
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Hartmann, Robert
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Kimmel, Nils
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Kirian, Richard A.
(author)
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Seibert, M. MarvinUppsala universitet,Molekylär biofysik(Swepub:uu)masei255
(author)
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Mühlig, KerstinUppsala universitet,Molekylär biofysik(Swepub:uu)kermu539
(author)
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Schorb, Sebastian
(author)
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Ferguson, Ken
(author)
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Bostedt, Christoph
(author)
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Carron, Sebastian
(author)
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Bozek, John D.
(author)
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Rolles, Daniel
(author)
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Rudenko, Artem
(author)
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Epp, Sascha
(author)
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Chapman, Henry N.
(author)
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Barty, Anton
(author)
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Hajdu, JanosUppsala universitet,Molekylär biofysik(Swepub:uu)janohajd
(author)
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Andersson, IngerUppsala universitet,Molekylär biofysik(Swepub:uu)inand172
(author)
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Uppsala universitetMolekylär biofysik
(creator_code:org_t)
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In:Nature Photonics: Springer Science and Business Media LLC8:12, s. 943-9491749-48851749-4893
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Hantke, Max F.
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Hasse, Dirk
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Maia, Filipe R. ...
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Ekeberg, Tomas
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John, Katja
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Svenda, Martin
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Loh, N. Duane
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Martin, Andrew V ...
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Timneanu, Nicuso ...
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Larsson, Daniel ...
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Gijs, van der Sc ...
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Carlsson, Gunill ...
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Ingelman, Margar ...
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Andreasson, Jako ...
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Westphal, Daniel
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Liang, Mengning
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Stellato, France ...
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DePonte, Daniel ...
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Hartmann, Robert
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Kimmel, Nils
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Kirian, Richard ...
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Seibert, M. Marv ...
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Mühlig, Kerstin
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Schorb, Sebastia ...
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Ferguson, Ken
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Bostedt, Christo ...
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Carron, Sebastia ...
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Bozek, John D.
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Rolles, Daniel
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Rudenko, Artem
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Epp, Sascha
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Chapman, Henry N ...
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Barty, Anton
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Hajdu, Janos
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Andersson, Inger
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- NATURAL SCIENCES
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NATURAL SCIENCES
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Nature Photonics
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Uppsala University