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Sensitive detection...
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Hammond, MariaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
(author)
Sensitive detection of aggregated prion protein via proximity ligation
- Article/chapterEnglish2014
Publisher, publication year, extent ...
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2014-11
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Informa UK Limited,2014
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-239529
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-239529URI
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https://doi.org/10.4161/pri.32231DOI
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https://res.slu.se/id/publ/67818URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of three or more copies of the specific protein. We have developed a SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at hundred-fold higher concentration. Using either of the two monoclonal anti-PrP antibodies 3F4 and 6H4 we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Wik, LottaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)lowik226
(author)
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Deslys, Jean-Philippe
(author)
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Comoy, Emmanuel
(author)
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Linne, TommySwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)47265
(author)
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Landegren, UlfUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)ulfland
(author)
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Kamali-Moghaddam, MasoodUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)masookam
(author)
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Uppsala universitetInstitutionen för immunologi, genetik och patologi
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Related titles
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In:Prion: Informa UK Limited8:3, s. 261-2651933-68961933-690X
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Prion
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