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- Hammond, MariaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab (author)
Sensitive detection of aggregated prion protein via proximity ligation
- Article/chapterEnglish2014
Publisher, publication year, extent ...
- 2014-11
- Informa UK Limited,2014
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-239529
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-239529URI
- https://doi.org/10.4161/pri.32231DOI
- https://res.slu.se/id/publ/67818URI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of three or more copies of the specific protein. We have developed a SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at hundred-fold higher concentration. Using either of the two monoclonal anti-PrP antibodies 3F4 and 6H4 we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.
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- Wik, LottaUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)lowik226 (author)
- Deslys, Jean-Philippe (author)
- Comoy, Emmanuel (author)
- Linne, TommySwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)47265 (author)
- Landegren, UlfUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)ulfland (author)
- Kamali-Moghaddam, MasoodUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)masookam (author)
- Uppsala universitetInstitutionen för immunologi, genetik och patologi (creator_code:org_t)
- Sveriges lantbruksuniversitet
Related titles
- In:Prion: Informa UK Limited8:3, s. 261-2651933-68961933-690X
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