Dihydroxyacetone-induced oscillations in cytoplasmic free Ca2+ and the ATP/ADP ratio in pancreatic beta-cells at substimulatory glucose.

Webb, Dominic-Luc (author): Department of Molecular Medicine, The Rolf Luft Center for Diabetes Research, Karolinska Institutet, Stockholm

Arkhammar, Per O G (author)

Schultz, Vera (author)

Schweda, Elke K H (author): Södertörns högskola,Karolinska Institutet,Avdelning Naturvetenskap

Tornheim, Keith (author)

(creator_code:org_t)

2003
2003
English.
In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 278:42, s. 40710-40716

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; http://kipublication...; https://urn.kb.se/re...; show less...

Abstract Subject headings

Glucose stimulation of pancreatic beta-cells causes oscillatory influx of Ca2+, leading to pulsatile insulin secretion. We have proposed that this is due to oscillations of glycolysis and the ATP/ADP ratio, which modulate the activity of ATP-sensitive K+ channels. We show here that dihydroxyacetone, a secretagogue that feeds into glycolysis below the putative oscillator phosphofructokinase, could cause a single initial peak in cytoplasmic free Ca2+ ([Ca2+]i) but did not by itself cause repeated oscillations in [Ca2+]i in mouse pancreatic beta-cells. However, in the presence of a substimulatory concentration of glucose (4 mm), dihydroxyacetone induced [Ca2+]i oscillations. Furthermore, these oscillations correlated with oscillations in the ATP/ADP ratio, as seen previously with glucose stimulation. Insulin secretion in response to dihydroxyacetone was transient in the absence of glucose but was considerably enhanced and somewhat prolonged in the presence of a substimulatory concentration of glucose, in accordance with the enhanced [Ca2+]i response. These results are consistent with the hypothesized role of phosphofructokinase as the generator of the oscillations. Dihydroxyacetone may affect phosphofructokinase by raising the free concentration of fructose 1,6-bisphosphate to a critical level at which it activates the enzyme autocatalytically, thereby inducing the pulses of phosphofructokinase activity that cause the metabolic oscillations.

By the author/editor: Juntti-Berggren, ...; Webb, Dominic-Lu ...; Arkhammar, Per O ...; Schultz, Vera; Schweda, Elke K ...; Tornheim, Keith; show more...; Berggren, Per-Ol ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

By the university: Uppsala University; Karolinska Institutet; Södertörn University

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