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A Transcriptome Der...
A Transcriptome Derived Female-Specific Marker from the Invasive Western Mosquitofish (Gambusia affinis)
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Lamatsch, Dunja K. (author)
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- Adolfsson, Sofia (author)
- Uppsala universitet,Evolutionsbiologi
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Senior, Alistair M. (author)
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Christiansen, Guntram (author)
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Pichler, Maria (author)
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Ozaki, Yuichi (author)
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- Smeds, Linnea (author)
- Uppsala universitet,Evolutionsbiologi
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Schartl, Manfred (author)
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Nakagawa, Shinichi (author)
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(creator_code:org_t)
- 2015-02-23
- 2015
- English.
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:2
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3' UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sex-specific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.
Subject headings
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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