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Photoinduced reduct...
Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme
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- Raleiras, Patrícia (author)
- Uppsala universitet,Molekylär biomimetik
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- Hammarström, Leif (author)
- Uppsala universitet,Fysikalisk kemi
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- Lindblad, Peter (author)
- Uppsala universitet,Molekylär biomimetik,Microbial chemistry
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- Styring, Stenbjörn (author)
- Uppsala universitet,Molekylär biomimetik
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- Magnuson, Ann (author)
- Uppsala universitet,Molekylär biomimetik
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Journal of Inorganic Biochemistry. - : Elsevier BV. - 0162-0134 .- 1873-3344. ; 148, s. 57-61
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The small subunit from the NiFe uptake hydrogenase, HupSL, in the cyanobacterium Nostoc punctiforme ATCC 29133, has been isolated in the absence of the large subunit (P. Raleiras, P. Kellers, P. Lindblad, S. Styring, A. Magnuson, J. Biol. Chem. 288 (2013) 18,345-18,352). Here, we have used flash photolysis to reduce the iron-sulfur clusters in the isolated small subunit, HupS. We used ascorbate as electron donor to the photogenerated excited state of Ru(II)-trisbipyridine (Ru(bpy)3), to generate Ru(I)(bpy)3 as reducing agent. Our results show that the isolated small subunit can be reduced by the Ru(I)(bpy)3 generated through flash photolysis.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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