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Influences on gene ...
Influences on gene expression in vivo by a Shine-Dalgarno sequence.
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- Jin, Haining (author)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Zhao, Qing (author)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Gonzalez de Valdivia, Ernesto I (author)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Ardell, David H (author)
- Uppsala universitet,Centrum för bioinformatik,Ardell
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- Stenström, Magnus (author)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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- Isaksson, Leif A (author)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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(creator_code:org_t)
- Wiley, 2006
- 2006
- English.
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In: Mol Microbiol. - : Wiley. - 0950-382X .- 1365-2958. ; 60:2, s. 480-92
- Related links:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The Shine-Dalgarno (SD+: 5'-AAGGAGG-3') sequence anchors the mRNA by basepairing to the 16S rRNA in the small ribosomal subunit during translation initiation. We have here comparedhow an SD+ sequence influences gene expression, if located upstream or downstream of an initiation codon.The positive effect of an upstream SD+ is confirmed. A downstream SD+ gives decreased gene expression.This effect is also valid for appropriately modified natural Escherichia coli genes. If an SD+ is placedbetween two potential initiation codons, initiation takes place predominantly at the second start site.The first start site is activated if the distance between this site and the downstream SD+ is enlargedand/or if the second start site is weakened. Upstream initiation is eliminated if a stable stem-loopstructure is placed between this SD+ and the upstream start site. The results suggest that the two startsites compete for ribosomes that bind to an SD+ located between them. A minor positive contribution toupstream initiation resulting from 3' to 5' ribosomal diffusion along the mRNA is suggested. Analysisof the E. coli K12 genome suggests that the SD+ or SD-like sequences are systematically avoided in theearly coding region suggesting an evolutionary significance.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Base Sequence
- Binding Sites
- Codon; Initiator/genetics/metabolism
- Escherichia coli/*genetics/metabolism
- Gene Expression Regulation; Bacterial
- Genes; Bacterial/genetics
- Genes; Reporter
- Molecular Sequence Data
- Nucleic Acid Conformation
- Protein Biosynthesis/*genetics
- RNA; Bacterial/metabolism
- RNA; Messenger/metabolism
- RNA; Ribosomal; 16S/metabolism
- RNA; Transfer; Amino Acyl/chemistry/metabolism
- Ribosomes/*metabolism
Publication and Content Type
- vet (subject category)
- art (subject category)
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