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Pyrylium Salts as R...
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Shariatgorji, Mohammadreza
(author)
Pyrylium Salts as Reactive Matrices for MALDI-MS Imaging of Biologically Active Primary Amines
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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2015-03-28
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American Chemical Society (ACS),2015
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-255057
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-255057URI
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https://doi.org/10.1007/s13361-015-1119-9DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:131235682URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Many neuroactive substances, including endogenous biomolecules, environmental compounds, and pharmaceuticals possess primary amine functional groups. Among these are catecholamine neurotransmitters (e.g., dopamine), many substituted phenethylamines (e.g., amphetamine), as well as amino acids and neuropeptides. In most cases, mass spectrometric (ESI and MALDI) analyses of trace amounts of such compounds are challenging because of their poor ionization properties. We present a method for chemical derivatization of primary amines by reaction with pyrylium salts that facilitates their detection by MALDI-MS and enables the imaging of primary amines in brain tissue sections. A screen of pyrylium salts revealed that the 2,4-diphenyl-pyranylium ion efficiently derivatizes primary amines and can be used as a reactive MALDI-MS matrix that induces both derivatization and desorption. MALDI-MS imaging with such matrix was used to map the localization of dopamine and amphetamine in brain tissue sections and to quantitatively map the distribution of the neurotoxin beta-N-methylamino-L-alanine.
Subject headings and genre
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Nilsson, Anna
(author)
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Kallback, Patrik
(author)
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Karlsson, OskarKarolinska Institutet,Uppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)oskar255
(author)
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Zhang, XiaoqunKarolinska Institutet
(author)
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Svenningsson, PerKarolinska Institutet
(author)
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Andren, Per E.
(author)
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Uppsala universitetInstitutionen för farmaceutisk biovetenskap
(creator_code:org_t)
Related titles
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In:Journal of the American Society for Mass Spectrometry: American Chemical Society (ACS)26:6, s. 934-9391044-03051879-1123
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