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Auxotrophy and intr...
Auxotrophy and intrapopulation complementary in the "interactome' of a cultivated freshwater model community
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- Garcia, Sarahi L. (author)
- Uppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab
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- Buck, Moritz (author)
- Uppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab
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- McMahon, Katherine D. (author)
- Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA.;Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA.
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- Grossart, Hans-Peter (author)
- Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Expt Limnol, D-16775 Ot Neuglobsow, Stechlin, Germany.;Univ Potsdam, Inst Biochem & Biol, D-14476 Potsdam, Germany.
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- Eiler, Alexander (author)
- Uppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab
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- Warnecke, Falk (author)
- Univ Jena, Jena Sch Microbial Commun, D-07743 Jena, Germany.
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(creator_code:org_t)
- 2015-08-08
- 2015
- English.
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In: Molecular Ecology. - : Wiley. - 0962-1083 .- 1365-294X. ; 24:17, s. 4449-4459
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Microorganisms are usually studied either in highly complex natural communities or in isolation as monoclonal model populations that we manage to grow in the laboratory. Here, we uncover the biology of some of the most common and yet-uncultured bacteria in freshwater environments using a mixed culture from Lake Grosse Fuchskuhle. From a single shotgun metagenome of a freshwater mixed culture of low complexity, we recovered four high-quality metagenome-assembled genomes (MAGs) for metabolic reconstruction. This analysis revealed the metabolic interconnectedness and niche partitioning of these naturally dominant bacteria. In particular, vitamin- and amino acid biosynthetic pathways were distributed unequally with a member of Crenarchaeota most likely being the sole producer of vitamin B12 in the mixed culture. Using coverage-based partitioning of the genes recovered from a single MAG intrapopulation metabolic complementarity was revealed pointing to social' interactions for the common good of populations dominating freshwater plankton. As such, our MAGs highlight the power of mixed cultures to extract naturally occurring interactomes' and to overcome our inability to isolate and grow the microbes dominating in nature.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- community
- cultures
- interactions
- metagenomics
- populations
Publication and Content Type
- ref (subject category)
- art (subject category)
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