Search: onr:"swepub:oai:DiVA.org:uu-263539" >
Electronic damage i...
Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse
-
- Galli, L. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.;Univ Hamburg, Dept Phys, D-20355 Hamburg, Germany.
-
Son, S. -K (author)
-
- Klinge, M. (author)
- Univ Hamburg, Inst Biochem & Mol Biol, Joint Lab Struct Biol Infect & Inflammat, D-22607 Hamburg, Germany.;Univ Lubeck, Inst Biochem, DESY, D-22607 Hamburg, Germany.
-
show more...
-
- Bajt, S. (author)
- Deutsch Elektronen Synchrotron DESY, Photon Sci, D-22607 Hamburg, Germany.
-
- Barty, A. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Bean, R. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Betzel, C. (author)
- Univ Hamburg, DESY, Inst Biochem & Mol Biol, Dept Chem, D-22607 Hamburg, Germany.
-
- Beyerlein, K. R. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Caleman, Carl (author)
- Uppsala universitet,Molekyl- och kondenserade materiens fysik,Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Doak, R. B. (author)
- Max Planck Inst Med Res, Dept Biomol Mech, D-69120 Heidelberg, Germany.
-
- Duszenko, M. (author)
- Univ Tubingen, Interfac Inst Biochem, D-72076 Tubingen, Germany.
-
- Fleckenstein, H. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Gati, C. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Hunt, B. (author)
- Brigham Young Univ, Dept Phys & Astron, Provo, UT 84602 USA.
-
- Kirian, R. A. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Liang, M. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Nanao, M. H. (author)
- EMBL, Grenoble Outstn, F-38042 Grenoble, France.
-
- Nass, K. (author)
- Max Planck Inst Med Res, Dept Biomol Mech, D-69120 Heidelberg, Germany.
-
- Oberthuer, D. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Redecke, L. (author)
- Univ Hamburg, Inst Biochem & Mol Biol, Joint Lab Struct Biol Infect & Inflammat, D-22607 Hamburg, Germany.;Univ Lubeck, Inst Biochem, DESY, D-22607 Hamburg, Germany.
-
- Shoeman, R. (author)
- Max Planck Inst Med Res, Dept Biomol Mech, D-69120 Heidelberg, Germany.
-
- Stellato, F. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Yoon, C. H. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.;European XFEL GmbH, D-22761 Hamburg, Germany.
-
- White, T. A. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Yefanov, O. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.
-
- Spence, J. (author)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA.
-
- Chapman, H. N. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany.;Univ Hamburg, Dept Phys, D-20355 Hamburg, Germany.;Hamburg Ctr Ultrafast Imaging, D-22761 Hamburg, Germany.
-
show less...
-
Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany;Univ Hamburg, Dept Phys, D-20355 Hamburg, Germany. Univ Hamburg, Inst Biochem & Mol Biol, Joint Lab Struct Biol Infect & Inflammat, D-22607 Hamburg, Germany.;Univ Lubeck, Inst Biochem, DESY, D-22607 Hamburg, Germany. (creator_code:org_t)
- AIP Publishing, 2015
- 2015
- English.
-
In: Structural Dynamics. - : AIP Publishing. - 2329-7778. ; 2:4
- Related links:
-
https://uu.diva-port... (primary) (Raw object)
-
show more...
-
https://aca.scitatio...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Current hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their electrons. This electronic radiation damage causes the atomic scattering factors to change, affecting, in particular, the heavy atoms, due to their higher photoabsorption cross sections. Here, it is shown that experimental serial femtosecond crystallography data collected with an extremely bright XFEL source exhibit a reduction of the effective scattering power of the sulfur atoms in a native protein. Quantitative methods are developed to retrieve information on the effective ionization of the damaged atomic species from experimental data, and the implications of utilizing new phasing methods which can take advantage of this localized radiation damage are discussed.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Galli, L.
-
Son, S. -K
-
Klinge, M.
-
Bajt, S.
-
Barty, A.
-
Bean, R.
-
show more...
-
Betzel, C.
-
Beyerlein, K. R.
-
Caleman, Carl
-
Doak, R. B.
-
Duszenko, M.
-
Fleckenstein, H.
-
Gati, C.
-
Hunt, B.
-
Kirian, R. A.
-
Liang, M.
-
Nanao, M. H.
-
Nass, K.
-
Oberthuer, D.
-
Redecke, L.
-
Shoeman, R.
-
Stellato, F.
-
Yoon, C. H.
-
White, T. A.
-
Yefanov, O.
-
Spence, J.
-
Chapman, H. N.
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
- Articles in the publication
-
Structural Dynam ...
- By the university
-
Uppsala University