Search: onr:"swepub:oai:DiVA.org:uu-265822" >
Comparative Sigma F...
-
Pettersson, B. M. FredrikUppsala universitet,Kemisk biologi
(author)
Comparative Sigma Factor-mRNA Levels in Mycobacterium marinum under Stress Conditions and during Host Infection
- Article/chapterEnglish2015
Publisher, publication year, extent ...
-
2015-10-07
-
Public Library of Science (PLoS),2015
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-265822
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-265822URI
-
https://doi.org/10.1371/journal.pone.0139823DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
We have used RNASeq and qRT-PCR to study mRNA levels for all s-factors in different Mycobacterium marinum strains under various growth and stress conditions. We also studied their levels in M. marinum from infected fish and mosquito larvae. The annotated s-factors were expressed and transcripts varied in relation to growth and stress conditions. Some were highly abundant such as sigA, sigB, sigC, sigD, sigE and sigH while others were not. The s-factor mRNA profiles were similar after heat stress, during infection of fish and mosquito larvae. The similarity also applies to some of the known heat shock genes such as the a-crystallin gene. Therefore, it seems probable that the physiological state of M. marinum is similar when exposed to these different conditions. Moreover, the mosquito larvae data suggest that this is the state that the fish encounter when infected, at least with respect to s-factor mRNA levels. Comparative genomic analysis of s-factor gene localizations in three M. marinum strains and Mycobacterium tuberculosis H37Rv revealed chromosomal rearrangements that changed the localization of especially sigA, sigB, sigD, sigE, sigF and sigJ after the divergence of these two species. This may explain the variation in species-specific expression upon exposure to different growth conditions.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Das, SarbashisUppsala universitet,Kemisk biologi,Uppsala Univ, Biomed Ctr, Dept Cell & Mol Biol, Uppsala, Sweden.(Swepub:uu)sarda235
(author)
-
Behra, Phani Rama KrishnaUppsala universitet,Kemisk biologi(Swepub:uu)behph854
(author)
-
Jordan, Heather R.Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA.
(author)
-
Ramesh, MalavikaUppsala universitet,Kemisk biologi(Swepub:uu)malra274
(author)
-
Mallick, AmritaUniv Louisiana, Dept Biol, Lafayette, LA USA.
(author)
-
Root, Kate M.Univ Louisiana, Dept Biol, Lafayette, LA USA.
(author)
-
Cheramie, Martin N.Univ Louisiana, Dept Biol, Lafayette, LA USA.
(author)
-
Melara, Irma de la CruzUppsala universitet,Kemisk biologi,Uppsala Univ, Biomed Ctr, Dept Cell & Mol Biol, Uppsala, Sweden.
(author)
-
Small, Pamela L. C.Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA.
(author)
-
Dasgupta, SantanuUppsala universitet,Mikrobiologi,Uppsala Univ, Biomed Ctr, Dept Cell & Mol Biol, Uppsala, Sweden.(Swepub:uu)sda03860
(author)
-
Ennis, Don G.Univ Louisiana, Dept Biol, Lafayette, LA USA.
(author)
-
Kirsebom, Leif A.Uppsala universitet,Kemisk biologi(Swepub:uu)leifkirs
(author)
-
Uppsala universitetKemisk biologi
(creator_code:org_t)
Related titles
-
In:PLOS ONE: Public Library of Science (PLoS)10:101932-6203
Internet link
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Pettersson, B. M ...
-
Das, Sarbashis
-
Behra, Phani Ram ...
-
Jordan, Heather ...
-
Ramesh, Malavika
-
Mallick, Amrita
-
show more...
-
Root, Kate M.
-
Cheramie, Martin ...
-
Melara, Irma de ...
-
Small, Pamela L. ...
-
Dasgupta, Santan ...
-
Ennis, Don G.
-
Kirsebom, Leif A ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Microbiology
- Articles in the publication
-
PLOS ONE
- By the university
-
Uppsala University