Search: onr:"swepub:oai:DiVA.org:uu-267034" >
NMR-based metabolic...
NMR-based metabolic profiling in healthy individuals overfed different types of fat : links to changes in liver fat accumulation and lean tissue mass.
-
- Elmsjö, Albert (author)
- Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi
-
- Rosqvist, Fredrik (author)
- Uppsala universitet,Klinisk nutrition och metabolism
-
- Engskog, Mikael K R (author)
- Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi
-
show more...
-
- Haglöf, Jakob (author)
- Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi
-
- Kullberg, Joel (author)
- Uppsala universitet,Radiologi
-
- Iggman, David (author)
- Uppsala universitet,Klinisk nutrition och metabolism
-
- Johansson, Lars (author)
- Uppsala universitet,Radiologi
-
- Ahlström, Håkan (author)
- Uppsala universitet,Radiologi
-
- Arvidsson, Torbjörn (author)
- Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi
-
- Risérus, Ulf (author)
- Uppsala universitet,Klinisk nutrition och metabolism
-
- Pettersson, Curt (author)
- Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi
-
show less...
-
(creator_code:org_t)
- 2015-10-19
- 2015
- English.
-
In: Nutrition & Diabetes. - : Springer Science and Business Media LLC. - 2044-4052. ; 5:19, s. e182-
- Related links:
-
https://uu.diva-port... (primary) (Raw object)
-
show more...
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- BACKGROUND: Overeating different dietary fatty acids influence the amount of liver fat stored during weight gain, however, the mechanisms responsible are unclear. We aimed to identify non-lipid metabolites that may differentiate between saturated (SFA) and polyunsaturated fatty acid (PUFA) overfeeding using a non-targeted metabolomic approach. We also investigated the possible relationships between plasma metabolites and body fat accumulation.METHODS: In a randomized study (LIPOGAIN study), n=39 healthy individuals were overfed with muffins containing SFA or PUFA. Plasma samples were precipitated with cold acetonitrile and analyzed by nuclear magnetic resonance (NMR) spectroscopy. Pattern recognition techniques were used to overview the data, identify variables contributing to group classification and to correlate metabolites with fat accumulation.RESULTS: We previously reported that SFA causes a greater accumulation of liver fat, visceral fat and total body fat, whereas lean tissue levels increases less compared with PUFA, despite comparable weight gain. In this study, lactate and acetate were identified as important contributors to group classification between SFA and PUFA (P<0.05). Furthermore, the fat depots (total body fat, visceral adipose tissue and liver fat) and lean tissue correlated (P(corr)>0.5) all with two or more metabolites (for example, branched amino acids, alanine, acetate and lactate). The metabolite composition differed in a manner that may indicate higher insulin sensitivity after a diet with PUFA compared with SFA, but this needs to be confirmed in future studies.CONCLUSION: A non-lipid metabolic profiling approach only identified a few metabolites that differentiated between SFA and PUFA overfeeding. Whether these metabolite changes are involved in depot-specific fat storage and increased lean tissue mass during overeating needs further investigation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Näringslära (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Nutrition and Dietetics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Elmsjö, Albert
-
Rosqvist, Fredri ...
-
Engskog, Mikael ...
-
Haglöf, Jakob
-
Kullberg, Joel
-
Iggman, David
-
show more...
-
Johansson, Lars
-
Ahlström, Håkan
-
Arvidsson, Torbj ...
-
Risérus, Ulf
-
Pettersson, Curt
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Health Sciences
-
and Nutrition and Di ...
- Articles in the publication
-
Nutrition & Diab ...
- By the university
-
Uppsala University