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Challenges to get a...
Challenges to get axenic cultures of Trichomonas spp. : A new approach in eradication of contaminants and maintenance of laboratory microbiological cultures
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- Koptina, Anna (author)
- Uppsala universitet,Avdelningen för farmakognosi,Volga State Univ Technol, Yoshkar Ola 424000, Russia.
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- Strese, Åke (author)
- Uppsala universitet,Avdelningen för farmakognosi
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- Backlund, Anders (author)
- Uppsala universitet,Avdelningen för farmakognosi
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- Alsmark, Cecilia (author)
- Uppsala universitet,Avdelningen för farmakognosi,Natl Vet Inst SVA, Dept Virol Immunobiol & Parasitol, S-75651 Uppsala, Sweden.
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012 .- 1872-8359. ; 118, s. 25-30
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Contamination of microbiological and cell cultures is a major problem in many scientific and clinical laboratories as well as bioproduct manufacturers worldwide. In the current study we established a rapid (9 day) method to detect and eliminate fungal and bacterial contamination in cultures of the unicellular eukaryote Trichomonas spp. The developed method combines identification of the contaminating microorganisms using PCR and sequencing of the 16/18S regions followed by phylogenetic analysis. The next step was a phylogeny-guided selection of antibiotic treatments. We then used a two-step propidium iodide-resorufin assay to test the effect of selected antibiotics. The result was a quick and worthwhile purification of trichomonad laboratory cultures. Our workflow may also be implemented to obtain new isolates of trichomonads from clinical samples if initial broad-spectrum antibiotic therapy fails.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- Trichomonas spp.
- Contamination
- Resorufin
- Propidium iodide
- Antibiotic
- Phylogeny
Publication and Content Type
- ref (subject category)
- art (subject category)
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