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Knockdown of Tmem23...
Knockdown of Tmem234 in zebrafish results in proteinuria
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- Rodriguez, Patricia Q. (author)
- Karolinska Institutet
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- Oddsson, Asmundur (author)
- Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden.
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- Ebarasi, Lwaki (author)
- Karolinska Institutet
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- He, Bing (author)
- Karolinska Institutet
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- Hultenby, Kjell (author)
- Karolinska Institutet
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- Wernerson, Annika (author)
- Karolinska Institutet
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- Betsholtz, Christer (author)
- Karolinska Institutet,Uppsala universitet,Vaskulärbiologi,Karolinska Inst, Dept Med Biochem & Biophys, Div Vasc Biol, S-17177 Stockholm, Sweden.
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- Tryggvason, Karl (author)
- Karolinska Institutet
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- Patrakka, Jaakko (author)
- Karolinska Institutet
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Karolinska Institutet Karolinska Inst, Dept Med Biochem & Biophys, Div Matrix Biol, S-17177 Stockholm, Sweden (creator_code:org_t)
- American Physiological Society, 2015
- 2015
- English.
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In: AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY. - : American Physiological Society. - 1931-857X .- 1522-1466. ; 309:11, s. F955-F966
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https://www.physiolo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Podocytes are highly specialized epithelial cells located at the outer aspects of the glomerular capillary tuft and critical components of the kidney filtration barrier. To maintain their unique features, podocytes express a number of proteins that are only sparsely found elsewhere in the body. In this study, we have identified four (Tmem234, Znf185, Lrrc49, and Slfn5) new highly podocyte-enriched proteins. The proteins are strongly expressed by podocytes, while other parts of the kidney show only weak or no expression. Tmem234, Slfn5, and Lrrc49 are located in foot processes, whereas Znf185 is found in both foot and major processes. Expressional studies in developing kidneys show that these proteins are first expressed at the capillary stage glomerulus, the same stage when the formation of major and foot processes begins. We identified zebrafish orthologs for Tmem234 and Znf185 genes and knocked down their expression using morpholino technology. Studies in zebrafish larvae indicate that Tmem234 is essential for the organization and functional integrity of the pronephric glomerulus filtration barrier, as inactivation of Tmem234 expression results in foot process effacement and proteinuria. In summary, we have identified four novel highly podocyte - enriched proteins and show that one of them, Tmem234, is essential for the normal filtration barrier in the zebrafish pronephric glomerulus. Identification of new molecular components of the kidney filtration barrier opens up possibilities to study their role in glomerulus biology and diseases.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- podocyte
- pronephros
- podocin
- Tmem234
Publication and Content Type
- ref (subject category)
- art (subject category)
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